UniProt Accession

 OTU1_YEAST; P43558; D6VTI6 

Genbank Protein ID

 D50617; BK006940 

Genbank Nucleotide ID

 BAA09197.1; DAA12396.1 

Protein Name

 Ubiquitin thioesterase OTU1 

Protein Synonyms/Alias

 OTU domain-containing protein 1 

Gene Name

 OTU1 

Gene Synonyms/Alias

 YFL044C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
160	FNDAILDKPNKDYAQ	ubiquitination	[1]
267	GYSFNTHKAQIKCNT	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression. 

Sequence Annotation

 DOMAIN 109 229 OTU.
 ZN_FING 270 294 C2H2-type.
 ACT_SITE 117 117 By similarity.
 ACT_SITE 120 120 Nucleophile (Probable).
 ACT_SITE 294 294 By similarity.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nucleus; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016579; P:protein deubiquitination; IDA:SGD.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IMP:SGD. 

Interpro

 IPR003323; OTU.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 

Pfam

 PF02338; OTU 

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS50802; OTU
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS