UniProt Accession

 PTTG1_RAT; P97613 

Genbank Protein ID

 U73030; AF021802 

Genbank Nucleotide ID

 AAB47974.1; AAC40036.1 

Protein Name

 Securin 

Protein Synonyms/Alias

 Pituitary tumor-transforming gene 1 protein 

Gene Name

 Pttg1 

Gene Synonyms/Alias

 Pttg 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
31	LKLGSGVKALDGKLQ	acetylation	[1]
70	TVNRVTEKPVKSSKP	acetylation	[1]
76	EKPVKSSKPLQSKQP	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (By similarity). 

Sequence Annotation

 MOTIF 58 61 D-box.
 MOTIF 68 70 TEK-box 1.
 MOTIF 91 93 TEK-box 2.
 MOTIF 179 192 SH3-binding.
 MOD_RES 95 95 Phosphothreonine (By similarity).
 MOD_RES 162 162 Phosphoserine; by CDK1 (By similarity).  

Keyword

 Cell cycle; Cell division; Chromosome partition; Complete proteome; Cytoplasm; DNA damage; DNA repair; Mitosis; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Repeat; SH3-binding; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 199 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:Compara.
 GO:0043022; F:ribosome binding; IPI:RGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051276; P:chromosome organization; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0045143; P:homologous chromosome segregation; IEA:Compara.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; IMP:RGD.
 GO:0001558; P:regulation of cell growth; IMP:RGD. 

Interpro

 IPR018008; Securin_separation-inh_met.
 IPR006940; Securin_separation_inhibitor. 

Pfam

 PF04856; Securin 

SMART

  

PROSITE

  

PRINTS