CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002827
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate synthase [NADPH] small chain 
Protein Synonyms/Alias
 Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT 
Gene Name
 gltD 
Gene Synonyms/Alias
 aspB; b3213; JW3180 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
25KKPLKIRKIEFVEIYacetylation[1]
41PFSEGQAKAQADRCLacetylation[1]
70NYIPNWLKLANEGRIacetylation[1]
129IERYINDKAFEMGWRacetylation[1]
143RPDMSGVKQTGKKVAacetylation[1]
171VLTRNGVKAVVFDRHacetylation[1]
288PFVSMEGKRVVVLGGacetylation[1]
343REEGVEFKFNVQPLGacetylation[1]
358IEVNGNGKVSGVKMVacetylation[1]
363NGKVSGVKMVRTEMGacetylation[1]
375EMGEPDAKGRRRAEIacetylation[1]
438AFQTSNPKIFAGGDIacetylation[1]
461TAIAEGRKAADGIMNacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 DOMAIN 38 69 4Fe-4S ferredoxin-type.
 METAL 94 94 Iron-sulfur (4Fe-4S) (Potential).
 METAL 98 98 Iron-sulfur (4Fe-4S) (Potential).
 METAL 104 104 Iron-sulfur (4Fe-4S) (Potential).
 METAL 108 108 Iron-sulfur (4Fe-4S) (Potential).  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Complete proteome; Direct protein sequencing; Glutamate biosynthesis; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 472 AA 
Protein Sequence
MSQNVYQFID LQRVDPPKKP LKIRKIEFVE IYEPFSEGQA KAQADRCLSC GNPYCEWKCP 60
VHNYIPNWLK LANEGRIFEA AELSHQTNTL PEVCGRVCPQ DRLCEGSCTL NDEFGAVTIG 120
NIERYINDKA FEMGWRPDMS GVKQTGKKVA IIGAGPAGLA CADVLTRNGV KAVVFDRHPE 180
IGGLLTFGIP AFKLEKEVMT RRREIFTGMG IEFKLNTEVG RDVQLDDLLS DYDAVFLGVG 240
TYQSMRGGLE NEDADGVYAA LPFLIANTKQ LMGFGETRDE PFVSMEGKRV VVLGGGDTAM 300
DCVRTSVRQG AKHVTCAYRR DEENMPGSRR EVKNAREEGV EFKFNVQPLG IEVNGNGKVS 360
GVKMVRTEMG EPDAKGRRRA EIVAGSEHIV PADAVIMAFG FRPHNMEWLA KHSVELDSQG 420
RIIAPEGSDN AFQTSNPKIF AGGDIVRGSD LVVTAIAEGR KAADGIMNWL EV 472 
Gene Ontology
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0004355; F:glutamate synthase (NADPH) activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006537; P:glutamate biosynthetic process; IDA:EcoCyc.
 GO:0097054; P:L-glutamate biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR017896; 4Fe4S_Fe-S-bd.
 IPR012285; Fum_reductase_C.
 IPR006006; Glut_synth_ssu2.
 IPR009051; Helical_ferredxn.
 IPR016040; NAD(P)-bd_dom.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 
Pfam
 PF00070; Pyr_redox 
SMART
  
PROSITE
 PS51379; 4FE4S_FER_2 
PRINTS