| Tag | Content |
|---|
| CPLM ID | CPLM-002986 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Polyphosphate kinase |
Protein Synonyms/Alias | ATP-polyphosphate phosphotransferase; Polyphosphoric acid kinase |
Gene Name | ppk |
Gene Synonyms/Alias | b2501; JW2486 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 28 | VLQEAADKSNPLIER | acetylation | [1] | | 51 | NNLDEFYKVRFAELK | acetylation | [1] | | 79 | HSRHLLGKIQSRVLK | acetylation | [1, 2] | | 128 | NWLRHYFKQYLRQHI | acetylation | [1] | | 178 | LLEIPSDKVPRFVNL | acetylation | [1] | | 298 | GGRYHNFKDFINFPN | acetylation | [1] | | 326 | LRHIWFDKAQFRNGF | acetylation | [1] | | 416 | EANIHWAKRLTEAGV | acetylation | [1] | | 437 | PGLKIHAKLFLISRK | acetylation | [1] | | 444 | KLFLISRKENGEVVR | acetylation | [1] | | 463 | GTGNFNEKTARLYTD | acetylation | [1] | | 543 | KLNNLVDKGLVDRLY | acetylation | [1] | | 604 | IFENGGDKKVYLSSA | acetylation | [1] | | 656 | VKARYIDKELSNRYV | acetylation | [1] | | 682 | LAIYDYIKSLEQPE* | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli. Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z. J Proteome Res. 2013 Feb 1;12(2):844-51. [ PMID: 23294111] |
Functional Description | Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can also act in the reverse direction to form ATP in the presence of excess ADP. Can also use GTP instead of ATP; but the efficiency of GTP is 5% that of ATP. |
Sequence Annotation | DOMAIN 430 464 PLD phosphodiesterase.
ACT_SITE 435 435 Phosphohistidine intermediate.
ACT_SITE 454 454 Phosphohistidine intermediate. |
Keyword | 3D-structure; ATP-binding; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 688 AA |
Protein Sequence | MGQEKLYIEK ELSWLSFNER VLQEAADKSN PLIERMRFLG IYSNNLDEFY KVRFAELKRR 60
IIISEEQGSN SHSRHLLGKI QSRVLKADQE FDGLYNELLL EMARNQIFLI NERQLSVNQQ 120
NWLRHYFKQY LRQHITPILI NPDTDLVQFL KDDYTYLAVE IIRGDTIRYA LLEIPSDKVP 180
RFVNLPPEAP RRRKPMILLD NILRYCLDDI FKGFFDYDAL NAYSMKMTRD AEYDLVHEME 240
ASLMELMSSS LKQRLTAEPV RFVYQRDMPN ALVEVLREKL TISRYDSIVP GGRYHNFKDF 300
INFPNVGKAN LVNKPLPRLR HIWFDKAQFR NGFDAIRERD VLLYYPYHTF EHVLELLRQA 360
SFDPSVLAIK INIYRVAKDS RIIDSMIHAA HNGKKVTVVV ELQARFDEEA NIHWAKRLTE 420
AGVHVIFSAP GLKIHAKLFL ISRKENGEVV RYAHIGTGNF NEKTARLYTD YSLLTADARI 480
TNEVRRVFNF IENPYRPVTF DYLMVSPQNS RRLLYEMVDR EIANAQQGLP SGITLKLNNL 540
VDKGLVDRLY AASSSGVPVN LLVRGMCSLI PNLEGISDNI RAISIVDRYL EHDRVYIFEN 600
GGDKKVYLSS ADWMTRNIDY RIEVATPLLD PRLKQRVLDI IDILFSDTVK ARYIDKELSN 660
RYVPRGNRRK VRAQLAIYDY IKSLEQPE 688 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0009358; C:polyphosphate kinase complex; IEA:InterPro. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0008976; F:polyphosphate kinase activity; IEA:HAMAP. GO:0006799; P:polyphosphate biosynthetic process; IEA:HAMAP. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |