CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017694
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Biorientation of chromosomes in cell division protein 1-like 1 
Protein Synonyms/Alias
 Protein FAM44A 
Gene Name
 BOD1L1 
Gene Synonyms/Alias
 BOD1L; FAM44A; KIAA1327 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
81CLADVDTKPAYQNLRacetylation[1]
81CLADVDTKPAYQNLRubiquitination[1, 2, 3, 4]
473VRHAYVHKPYLYSKYacetylation[1, 5, 6, 7]
701KSTLKNEKHLKKDDSacetylation[1]
1942SGTEKGSKDTDICSSubiquitination[8]
1951TDICSSAKGIVESSVubiquitination[8]
2430PSAVCAEKEEKHGKEacetylation[5]
2981VSDPVEDKKEQESDEubiquitination[9, 10]
2982SDPVEDKKEQESDEEubiquitination[9, 10]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [8] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [9] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [10] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 DNA_BIND 2872 2884 A.T hook.
 MOD_RES 266 266 Phosphoserine.
 MOD_RES 473 473 N6-acetyllysine.
 MOD_RES 482 482 Phosphoserine.
 MOD_RES 484 484 Phosphoserine.
 MOD_RES 635 635 Phosphoserine.
 MOD_RES 1145 1145 Phosphoserine.
 MOD_RES 1354 1354 Phosphothreonine.
 MOD_RES 1531 1531 Phosphoserine.
 MOD_RES 1710 1710 Phosphoserine.
 MOD_RES 2501 2501 Phosphoserine.
 MOD_RES 2779 2779 Phosphoserine.
 MOD_RES 2780 2780 Phosphoserine.
 MOD_RES 2905 2905 Phosphoserine.
 MOD_RES 2907 2907 Phosphoserine.
 MOD_RES 2954 2954 Phosphoserine.
 MOD_RES 2956 2956 Phosphothreonine.
 MOD_RES 2958 2958 Phosphoserine.
 MOD_RES 2964 2964 Phosphoserine.
 MOD_RES 2973 2973 Phosphoserine.
 MOD_RES 2986 2986 Phosphoserine.
 CROSSLNK 2981 2981 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 2982 2982 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Complete proteome; Isopeptide bond; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 3051 AA 
Protein Sequence
MATNPQPQPP PPAPPPPPPQ PQPQPPPPPP GPGAGPGAGG AGGAGAGAGD PQLVAMIVNH 60
LKSQGLFDQF RRDCLADVDT KPAYQNLRQR VDNFVANHLA THTWSPHLNK NQLRNNIRQQ 120
VLKSGMLESG IDRIISQVVD PKINHTFRPQ VEKAVHEFLA TLNHKEEGSG NTAPDDEKPD 180
TSLITQGVPT PGPSANVAND AMSILETITS LNQEASAARA STETSNAKTS ERASKKLPSQ 240
PTTDTSTDKE RTSEDMADKE KSTADSGGEG LETAPKSEEF SDLPCPVEEI KNYTKEHNNL 300
ILLNKDVQQE SSEQKNKSTD KGEKKPDSNE KGERKKEKKE KTEKKFDHSK KSEDTQKVKD 360
EKQAKEKEVE SLKLPSEKNS NKAKTVEGTK EDFSLIDSDV DGLTDITVSS VHTSDLSSFE 420
EDTEEEVVTS DSMEEGEITS DDEEKNKQNK TKTQTSDSSE GKTKSVRHAY VHKPYLYSKY 480
YSDSDDELTV EQRRQSIAKE KEERLLRRQI NREKLEEKRK QKAEKTKSSK TKGQGRSSVD 540
LEESSTKSLE PKAARIKEVL KERKVLEKKV ALSKKRKKDS RNVEENSKKK QQYEEDSKET 600
LKTSEHCEKE KISSSKELKH VHAKSEPSKP ARRLSESLHV VDENKNESKL EREHKRRTST 660
PVIMEGVQEE TDTRDVKRQV ERSEICTEEP QKQKSTLKNE KHLKKDDSET PHLKSLLKKE 720
VKSSKEKPER EKTPSEDKLS VKHKYKGDCM HKTGDETELH SSEKGLKVEE NIQKQSQQTK 780
LSSDDKTERK SKHRNERKLS VLGKDGKPVS EYIIKTDENV RKENNKKERR LSAEKTKAEH 840
KSRRSSDSKI QKDSLGSKQH GITLQRRSES YSEDKCDMDS TNMDSNLKPE EVVHKEKRRT 900
KSLLEEKLVL KSKSKTQGKQ VKVVETELQE GATKQATTPK PDKEKNTEEN DSEKQRKSKV 960
EDKPFEETGV EPVLETASSS AHSTQKDSSH RAKLPLAKEK YKSDKDSTST RLERKLSDGH 1020
KSRSLKHSSK DIKKKDENKS DDKDGKEVDS SHEKARGNSS LMEKKLSRRL CENRRGSLSQ 1080
EMAKGEEKLA ANTLSTPSGS SLQRPKKSGD MTLIPEQEPM EIDSEPGVEN VFEVSKTQDN 1140
RNNNSQQDID SENMKQKTSA TVQKDELRTC TADSKATAPA YKPGRGTGVN SNSEKHADHR 1200
STLTKKMHIQ SAVSKMNPGE KEPIHRGTTE VNIDSETVHR MLLSAPSEND RVQKNLKNTA 1260
AEEHVAQGDA TLEHSTNLDS SPSLSSVTVV PLRESYDPDV IPLFDKRTVL EGSTASTSPA 1320
DHSALPNQSL TVRESEVLKT SDSKEGGEGF TVDTPAKASI TSKRHIPEAH QATLLDGKQG 1380
KVIMPLGSKL TGVIVENENI TKEGGLVDMA KKENDLNAEP NLKQTIKATV ENGKKDGIAV 1440
DHVVGLNTEK YAETVKLKHK RSPGKVKDIS IDVERRNENS EVDTSAGSGS APSVLHQRNG 1500
QTEDVATGPR RAEKTSVATS TEGKDKDVTL SPVKAGPATT TSSETRQSEV ALPCTSIEAD 1560
EGLIIGTHSR NNPLHVGAEA SECTVFAAAE EGGAVVTEGF AESETFLTST KEGESGECAV 1620
AESEDRAADL LAVHAVKIEA NVNSVVTEEK DDAVTSAGSE EKCDGSLSRD SEIVEGTITF 1680
ISEVESDGAV TSAGTEIRAG SISSEEVDGS QGNMMRMGPK KETEGTVTCT GAEGRSDNFV 1740
ICSVTGAGPR EERMVTGAGV VLGDNDAPPG TSASQEGDGS VNDGTEGESA VTSTGITEDG 1800
EGPASCTGSE DSSEGFAISS ESEENGESAM DSTVAKEGTN VPLVAAGPCD DEGIVTSTGA 1860
KEEDEEGEDV VTSTGRGNEI GHASTCTGLG EESEGVLICE SAEGDSQIGT VVEHVEAEAG 1920
AAIMNANENN VDSMSGTEKG SKDTDICSSA KGIVESSVTS AVSGKDEVTP VPGGCEGPMT 1980
SAASDQSDSQ LEKVEDTTIS TGLVGGSYDV LVSGEVPECE VAHTSPSEKE DEDIITSVEN 2040
EECDGLMATT ASGDITNQNS LAGGKNQGKV LIISTSTTND YTPQVSAITD VEGGLSDALR 2100
TEENMEGTRV TTEEFEAPMP SAVSGDDSQL TASRSEEKDE CAMISTSIGE EFELPISSAT 2160
TIKCAESLQP VAAAVEERAT GPVLISTADF EGPMPSAPPE AESPLASTSK EEKDECALIS 2220
TSIAEECEAS VSGVVVESEN ERAGTVMEEK DGSGIISTSS VEDCEGPVSS AVPQEEGDPS 2280
VTPAEEMGDT AMISTSTSEG CEAVMIGAVL QDEDRLTITR VEDLSDAAII STSTAECMPI 2340
SASIDRHEEN QLTADNPEGN GDLSATEVSK HKVPMPSLIA ENNCRCPGPV RGGKEPGPVL 2400
AVSTEEGHNG PSVHKPSAGQ GHPSAVCAEK EEKHGKECPE IGPFAGRGQK ESTLHLINAE 2460
EKNVLLNSLQ KEDKSPETGT AGGSSTASYS AGRGLEGNAN SPAHLRGPEQ TSGQTAKDPS 2520
VSIRYLAAVN TGAIKADDMP PVQGTVAEHS FLPAEQQGSE DNLKTSTTKC ITGQESKIAP 2580
SHTMIPPATY SVALLAPKCE QDLTIKNDYS GKWTDQASAE KTGDDNSTRK SFPEEGDIMV 2640
TVSSEENVCD IGNEESPLNV LGGLKLKANL KMEAYVPSEE EKNGEILAPP ESLCGGKPSG 2700
IAELQREPLL VNESLNVENS GFRTNEEIHS ESYNKGEISS GRKDNAEAIS GHSVEADPKE 2760
VEEEERHMPK RKRKQHYLSS EDEPDDNPDV LDSRIETAQR QCPETEPHDT KEENSRDLEE 2820
LPKTSSETNS TTSRVMEEKD EYSSSETTGE KPEQNDDDTI KSQEEDQPII IKRKRGRPRK 2880
YPVETTLKMK DDSKTDTGIV TVEQSPSSSK LKVMQTDESN KETANLQERS ISNDDGEEKI 2940
VTSVRRRGRK PKRSLTVSDD AESSEPERKR QKSVSDPVED KKEQESDEEE EEEEEDEPSG 3000
ATTRSTTRSE AQRSKTQLSP SIKRKREVSP PGARTRGQQR VEEAPVKKAK R 3051 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro. 
Interpro
 IPR017956; AT_hook_DNA-bd_motif.
 IPR026955; Bod1_like. 
Pfam
  
SMART
 SM00384; AT_hook 
PROSITE
  
PRINTS