CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-034131
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Glyceraldehyde-3-phosphate dehydrogenase 
Protein Synonyms/Alias
  
Gene Name
 Gm7293 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
61GKFNGTVKAENGKLVacetylation[1, 2, 3]
61GKFNGTVKAENGKLVsuccinylation[2]
61GKFNGTVKAENGKLVubiquitination[4]
187HAITATQKTVDGPSGubiquitination[4]
252DLACRLEKPAKYDDIacetylation[5, 6]
252DLACRLEKPAKYDDIphosphoglycerylation[7]
252DLACRLEKPAKYDDIubiquitination[4]
255CRLEKPAKYDDIKKVacetylation[5, 6]
255CRLEKPAKYDDIKKVubiquitination[4]
260PAKYDDIKKVVKQASacetylation[6]
260PAKYDDIKKVVKQASubiquitination[4]
335LMAYMASKE******acetylation[2]
335LMAYMASKE******ubiquitination[4]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [6] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [7] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 336 AA 
Protein Sequence
MVKVGVNGFG RIGRLVTRAA FCSPHGKVEI VAINDPFIDL NYMVYMFQYD STHGKFNGTV 60
KAENGKLVIN GKPITVFQER DPTNIKCGEA GAEYVMESTG VFTTMEKAGA HLFVTGAKRV 120
IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH 180
AITATQKTVD GPSGKLWRDS RGAAQNIIPA PTDAAKAVSK VIPELNGKLT GMAFRVPTHN 240
VSVVDLACRL EKPAKYDDIK KVVKQASEGS LKGILGYTED QVVSCDFNSN SHSSTFDAGA 300
GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE 336 
Gene Ontology
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006006; P:glucose metabolic process; IEA:InterPro. 
Interpro
 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 
SMART
 SM00846; Gp_dh_N 
PROSITE
 PS00071; GAPDH 
PRINTS
 PR00078; G3PDHDRGNASE.