UniProt Accession

 KCY_HUMAN; P30085; B4DPU7; E9PGI8; Q53GB7; Q5SVZ0; Q96C07; Q9UBQ8; Q9UIA2 

Genbank Protein ID

 AF070416; AF259961; AF110643; AF087865; AF112216; AK223014; AK298502; AL513322; AL607122; AL607122; AL513322; BC014961 

Genbank Nucleotide ID

 AAF17709.1; AAG22609.1; AAD48583.1; AAP97174.1; AAF17204.1; BAD96734.1; BAG60709.1; CAI13471.1; CAI13471.1; CAI14972.1; CAI14972.1; AAH14961.1 

Protein Name

 UMP-CMP kinase 

Protein Synonyms/Alias

 Deoxycytidylate kinase; CK; dCMP kinase; Uridine monophosphate/cytidine monophosphate kinase; UMP/CMP kinase; UMP/CMPK 

Gene Name

 CMPK1 

Gene Synonyms/Alias

 CMK; CMPK; UCK; UMK; UMPK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
26	QCARIVEKYGYTHLS	ubiquitination	[1]
43	ELLRDERKNPDSQYG	acetylation	[2, 3]
43	ELLRDERKNPDSQYG	ubiquitination	[3, 4, 5, 6]
55	QYGELIEKYIKEGKI	acetylation	[2, 7]
55	QYGELIEKYIKEGKI	ubiquitination	[1]
58	ELIEKYIKEGKIVPV	ubiquitination	[1]
88	AANAQKNKFLIDGFP	ubiquitination	[1, 3, 6, 8]
106	DNLQGWNKTMDGKAD	ubiquitination	[1, 5, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. 

Sequence Annotation

 NP_BIND 13 18 ATP (By similarity).
 NP_BIND 61 63 NMP (By similarity).
 NP_BIND 93 96 NMP (By similarity).
 REGION 33 63 NMPbind.
 REGION 133 143 LID.
 BINDING 39 39 NMP (By similarity).
 BINDING 100 100 CMP (By similarity).
 BINDING 134 134 ATP (By similarity).
 BINDING 140 140 NMP (By similarity).
 BINDING 151 151 NMP (By similarity).
 BINDING 179 179 ATP; via carbonyl oxygen (By similarity).
 MOD_RES 55 55 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Nucleus; Pyrimidine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 196 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004127; F:cytidylate kinase activity; IEA:EC.
 GO:0033862; F:UMP kinase activity; IEA:Compara.
 GO:0004849; F:uridine kinase activity; TAS:ProtInc.
 GO:0046705; P:CDP biosynthetic process; IEA:Compara.
 GO:0006240; P:dCDP biosynthetic process; IEA:Compara.
 GO:0006227; P:dUDP biosynthetic process; IEA:Compara.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0022602; P:ovulation cycle process; IEA:Compara.
 GO:0018963; P:phthalate metabolic process; IEA:Compara.
 GO:0006225; P:UDP biosynthetic process; IEA:Compara. 

Interpro

 IPR000850; Adenylate_kin.
 IPR027417; P-loop_NTPase.
 IPR006266; UMP_CMP_kinase. 

Pfam

 PF00406; ADK 

SMART

  

PROSITE

 PS00113; ADENYLATE_KINASE 

PRINTS

 PR00094; ADENYLTKNASE.