UniProt Accession

 PHO84_YEAST; P25297; D6W0G2 

Genbank Protein ID

 D90346; Z49218; BK006946 

Genbank Nucleotide ID

 BAA14358.1; CAA89157.1; DAA09776.1 

Protein Name

 Inorganic phosphate transporter PHO84 

Protein Synonyms/Alias

  

Gene Name

 PHO84 

Gene Synonyms/Alias

 YML123C; YM7056.03C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
6	**MSSVNKDTIHVAE	ubiquitination	[1, 2, 3]
18	VAERSLHKEHLTEGG	ubiquitination	[4]
297	AQEQDGEKKIHDTSD	ubiquitination	[4, 5]
298	QEQDGEKKIHDTSDE	ubiquitination	[1, 2, 3]

Reference

 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [5] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149] 

Functional Description

 High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast. 

Sequence Annotation

 MOD_RES 302 302 Phosphothreonine.
 MOD_RES 303 303 Phosphoserine.
 MOD_RES 317 317 Phosphothreonine.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 579 579 Phosphoserine.
 CROSSLNK 6 6 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 298 298 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Isopeptide bond; Membrane; Phosphate transport; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 587 AA 

Protein Sequence

Gene Ontology

 GO:0005887; C:integral to plasma membrane; IDA:SGD.
 GO:0005315; F:inorganic phosphate transmembrane transporter activity; IDA:SGD.
 GO:0005384; F:manganese ion transmembrane transporter activity; IMP:SGD.
 GO:0006817; P:phosphate ion transport; IDA:SGD.
 GO:0006797; P:polyphosphate metabolic process; IMP:SGD. 

Interpro

 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt.
 IPR004738; Phos_permease.
 IPR005828; Sub_transporter.
 IPR005829; Sugar_transporter_CS. 

Pfam

 PF00083; Sugar_tr 

SMART

  

PROSITE

 PS50850; MFS
 PS00216; SUGAR_TRANSPORT_1
 PS00217; SUGAR_TRANSPORT_2 

PRINTS