UniProt Accession

 MAX_HUMAN; P61244; A6NH73; A8K265; A8K4G4; A8K824; P25912; P52163; Q14803; Q96CY8 

Genbank Protein ID

 M64240; M64240; X66867; X66867; X66867; X60287; AK290130; AK290929; AK292189; AK292630; AL139022; CH471061; CH471061; CH471061; CH471061; BC003525; BC004516; BC013669; BC027924 

Genbank Nucleotide ID

 AAA36200.1; AAA36201.1; CAA47337.1; CAA47338.1; CAA47339.1; CAA42827.1; BAF82819.1; BAF83618.1; BAF84878.1; BAF85319.1; EAW80899.1; EAW80901.1; EAW80903.1; EAW80904.1; AAH03525.1; AAH04516.1; AAH13669.1; AAH27924.1 

Protein Name

 Protein max 

Protein Synonyms/Alias

 Class D basic helix-loop-helix protein 4; bHLHd4; Myc-associated factor X 

Gene Name

 MAX 

Gene Synonyms/Alias

 BHLHD4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
40	RKRRDHIKDSFHSLR	acetylation	[1]
57	VPSLQGEKASRAQIL	ubiquitination	[2]
66	SRAQILDKATEYIQY	acetylation	[1]
153	PEEPQSRKKLRMEAS	acetylation	[1]
154	EEPQSRKKLRMEAS*	acetylation	[1]

Reference

 [1] Max is acetylated by p300 at several nuclear localization residues.
 Faiola F, Wu YT, Pan S, Zhang K, Farina A, Martinez E.
 Biochem J. 2007 May 1;403(3):397-407. [PMID: 17217336]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. 

Sequence Annotation

 DOMAIN 23 74 bHLH.
 REGION 81 102 Leucine-zipper.
 MOTIF 152 156 Nuclear localization signal.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 66 66 N6-acetyllysine (Probable).
 MOD_RES 153 153 N6-acetyllysine.
 MOD_RES 154 154 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 160 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0016605; C:PML body; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0071375; P:cellular response to peptide hormone stimulus; IEA:Compara.
 GO:0009267; P:cellular response to starvation; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0006461; P:protein complex assembly; IEA:Compara.
 GO:0048678; P:response to axon injury; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IEA:Compara.
 GO:0060041; P:retina development in camera-type eye; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 

Interpro

 IPR011598; bHLH_dom. 

Pfam

 PF00010; HLH 

SMART

 SM00353; HLH 

PROSITE

 PS50888; BHLH 

PRINTS