CPLM-023055

Genbank Nucleotide ID

Anaphase-promoting complex subunit 7

Protein Synonyms/Alias

APC7; Cyclosome subunit 7

Homo sapiens (Human)

Position	Peptide	Type	References
99	EYRNAVSKYTMALQQ	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
107	YTMALQQKKALSKTS	ubiquitination	[2]
115	KALSKTSKVRPSTGN	ubiquitination	[5]
139	LPSEIEVKYKMAECY	ubiquitination	[5]
153	YTMLKQDKDAIAILD	ubiquitination	[2, 5]
181	MLANLYKKAGQERPS	ubiquitination	[5]
193	RPSVTSYKEVLRQCP	ubiquitination	[2, 5]
263	STICSLEKKSLLRDN	acetylation	[8, 9, 10]
263	STICSLEKKSLLRDN	ubiquitination	[5]
264	TICSLEKKSLLRDNV	ubiquitination	[5]
288	YFRAGDNKNSVLKFE	ubiquitination	[2, 3, 6, 10]
293	DNKNSVLKFEQAQML	ubiquitination	[1, 2, 3, 5, 6, 7, 10]
306	MLDPYLIKGMDVYGY	ubiquitination	[2]
353	GCHSFYSKRYSRALY	ubiquitination	[5]
364	RALYLGAKAIQLNSN	ubiquitination	[2, 5, 11]
379	SVQALLLKGAALRNM	ubiquitination	[1, 2, 5, 7, 11, 12]
460	EDPVTQEKAKTLLDK	ubiquitination	[5]
462	PVTQEKAKTLLDKAL	ubiquitination	[5]
467	KAKTLLDKALTQRPD	ubiquitination	[1, 2, 5, 7, 12]
477	TQRPDYIKAVVKKAE	ubiquitination	[1, 2, 5, 7, 12]
482	YIKAVVKKAELLSRE	ubiquitination	[5]
491	ELLSREQKYEDGIAL	ubiquitination	[1, 2, 5, 7]
551	KSLEGMQKMEKEESP	ubiquitination	[2, 5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[11] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[12] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

REPEAT 77 110 TPR 1.
REPEAT 169 202 TPR 2.
REPEAT 271 304 TPR 3.
REPEAT 373 406 TPR 4.
REPEAT 441 475 TPR 5.
REPEAT 476 508 TPR 6.
REPEAT 509 542 TPR 7.

3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Mitosis; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005680; C:anaphase-promoting complex; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0070979; P:protein K11-linked ubiquitination; IDA:UniProtKB.

IPR013026; TPR-contain_dom.
IPR011990; TPR-like_helical.
IPR013105; TPR_2.
IPR019734; TPR_repeat.

PS50005; TPR
PS50293; TPR_REGION