CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033649
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae), isoform CRA_a 
Protein Synonyms/Alias
 DCN1-like protein 1 
Gene Name
 DCUN1D1 
Gene Synonyms/Alias
 hCG_17206 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
128KLKAQIPKMEQELKEubiquitination[1, 2, 3, 4, 5, 6]
134PKMEQELKEPGRFKDubiquitination[2, 3, 4, 5, 6, 7, 8]
176LVLNGRFKFLDLWNKubiquitination[2]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 244 AA 
Protein Sequence
MIFTQSSEKT AVSCLSQNDW KLDVATDNFF QNPELYIRES VKGSLDRKKL EQLYNRYKDP 60
QDENKIGIDG IQQFCDDLAL DPASISVLII AWKFRAATQC EFSKQEFMDG MTELGCDSIE 120
KLKAQIPKME QELKEPGRFK DFYQFTFNFA KNPGQKGLDL EMAIAYWNLV LNGRFKFLDL 180
WNKFLLEHHK RSIPKDTWNL LLDFSTMIAD DMSNYDEEGA WPVLIDDFVE FARPQIAGTK 240
STTV 244 
Gene Ontology
  
Interpro
 IPR014764; DCN-prot.
 IPR005176; PONY_dom. 
Pfam
 PF03556; Cullin_binding 
SMART
  
PROSITE
 PS51229; DCUN1 
PRINTS