UniProt Accession

 RS4_ECOLI; P0A7V8; P02354; Q2M6W1 

Genbank Protein ID

 X02543; U18997; U00096; AP009048; V00353; J01685 

Genbank Nucleotide ID

 CAA26394.1; AAA58094.1; AAC76321.1; BAE77995.1; CAA23645.1; AAA24576.1 

Protein Name

 30S ribosomal protein S4 

Protein Synonyms/Alias

  

Gene Name

 rpsD 

Gene Synonyms/Alias

 ramA; b3296; JW3258 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
22	EGTDLFLKSGVRAID	acetylation	[1]
77	RQFRNYYKEAARLKG	acetylation	[1, 2]
156	AKKQSRVKAALELAE	acetylation	[1]
156	AKKQSRVKAALELAE	pupylation	[3]
167	ELAEQREKPTWLEVD	acetylation	[1]
167	ELAEQREKPTWLEVD	pupylation	[3]
177	WLEVDAGKMEGTFKR	acetylation	[1, 4]
183	GKMEGTFKRKPERSD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 One of two assembly initiator proteins for the 30S subunit, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. 

Sequence Annotation

 DOMAIN 96 156 S4 RNA-binding.  

Keyword

 3D-structure; Antibiotic resistance; Complete proteome; Direct protein sequencing; Reference proteome; Repressor; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding; Transcription; Transcription regulation; Transcription termination; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 206 AA 

Protein Sequence

Gene Ontology

 GO:0015935; C:small ribosomal subunit; IEA:InterPro.
 GO:0048027; F:mRNA 5'-UTR binding; IDA:EcoCyc.
 GO:0019843; F:rRNA binding; IDA:EcoliWiki.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0000900; F:translation repressor activity, nucleic acid binding; IMP:EcoCyc.
 GO:0006353; P:DNA-dependent transcription, termination; IEA:UniProtKB-KW.
 GO:0045947; P:negative regulation of translational initiation; IDA:EcoCyc.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
 GO:0031564; P:transcription antitermination; IDA:EcoCyc.
 GO:0006412; P:translation; IEA:HAMAP. 

Interpro

 IPR022801; Ribosomal_S4/S9.
 IPR001912; Ribosomal_S4/S9_N.
 IPR005709; Ribosomal_S4_bac-type.
 IPR018079; Ribosomal_S4_CS.
 IPR002942; S4_RNA-bd. 

Pfam

 PF00163; Ribosomal_S4
 PF01479; S4 

SMART

 SM00363; S4 

PROSITE

 PS00632; RIBOSOMAL_S4
 PS50889; S4 

PRINTS