CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021870
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rab5 GDP/GTP exchange factor 
Protein Synonyms/Alias
 Rabex-5 
Gene Name
 Rabgef1 
Gene Synonyms/Alias
 Rabex5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
85AQSLTFSKFEEKKTNubiquitination[1]
103RKVTTVKKFFSASSRubiquitination[1]
170FLEAMPYKRDLSIEEubiquitination[1]
303KHIFNAIKITKNEPAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A (By similarity). Can act as a ubiquitin ligase (By similarity). 
Sequence Annotation
 DOMAIN 232 375 VPS9.
 ZN_FING 13 47 A20-type.
 REGION 1 74 Interaction with ubiquitinated proteins  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Endocytosis; Endosome; Metal-binding; Protein transport; Reference proteome; Transport; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 491 AA 
Protein Sequence
MSLKSERRGI HVDQSELLCK KGCGYYGNPA WQGFCSKCWR EEYHKARQRQ IQEDWELAER 60
LQREEEEAFA SSQSSQGAQS LTFSKFEEKK TNEKTRKVTT VKKFFSASSR AGSKKEIQEA 120
KAPSPSINRQ TSIETDRVTK EFIDFLKTFH KTGQEVYKQT KMFLEAMPYK RDLSIEEQSE 180
CTQDFYQNVA ERMQTRGKVP PEKVEKIMDQ IEKHIMTRLY KFVFCPETTD DEKKDLAIQK 240
RIRALHWVTP QMLCVPVNEE IPEVSDMVVK AITDIIEMDS KRVPRDKLAC ITRCSKHIFN 300
AIKITKNEPA SADDFLPTLI YIVLKGNPPR LQSNIQYITR FCNPSRLMTG EDGYYFTNLC 360
CAVAFIEKLD AQSLNLSQED FDRYMSGQTS PRKQESESWP PEACLGVKQM YKNLDLLSQL 420
NERQERIMNE AKKLEKDLID WTDGIAKEVQ DIVEKYPLEI KPPNQPLAAI DSENVENDKL 480
PPPLQPQVYA G 491 
Gene Ontology
 GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; IEA:UniProtKB-SubCell.
 GO:0031982; C:vesicle; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:MGI.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0050728; P:negative regulation of inflammatory response; IMP:MGI.
 GO:1900165; P:negative regulation of interleukin-6 secretion; IMP:MGI.
 GO:1900235; P:negative regulation of Kit signaling pathway; IMP:MGI.
 GO:0002686; P:negative regulation of leukocyte migration; IMP:MGI.
 GO:0043305; P:negative regulation of mast cell degranulation; IMP:MGI.
 GO:0001933; P:negative regulation of protein phosphorylation; IMP:MGI.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:MGI.
 GO:0048261; P:negative regulation of receptor-mediated endocytosis; IMP:MGI.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0060368; P:regulation of Fc receptor mediated stimulatory signaling pathway; IDA:MGI. 
Interpro
 IPR003123; VPS9.
 IPR013995; VPS9_subgr.
 IPR002653; Znf_A20. 
Pfam
 PF02204; VPS9
 PF01754; zf-A20 
SMART
 SM00167; VPS9
 SM00259; ZnF_A20 
PROSITE
 PS51205; VPS9
 PS51036; ZF_A20 
PRINTS