CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004182
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 cAMP-dependent protein kinase type II-alpha regulatory subunit 
Protein Synonyms/Alias
  
Gene Name
 PRKAR2A 
Gene Synonyms/Alias
 PKR2; PRKAR2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
161AMFERIVKADEHVIDubiquitination[1, 2]
190TYDILVTKDNQTRSVubiquitination[1, 2]
280IVDVIGEKIYKDGERubiquitination[1]
318RSRTKSNKDGGNQEVubiquitination[1]
345ELALVTNKPRAASAYubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 
Functional Description
 Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. 
Sequence Annotation
 NP_BIND 139 260 cAMP 1.
 NP_BIND 261 404 cAMP 2.
 REGION 2 138 Dimerization and phosphorylation.
 BINDING 208 208 cAMP 1.
 BINDING 217 217 cAMP 1.
 BINDING 338 338 cAMP 2.
 BINDING 347 347 cAMP 2.
 MOD_RES 2 2 N-acetylserine (Probable).
 MOD_RES 48 48 Phosphoserine.
 MOD_RES 54 54 Phosphothreonine.
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 78 78 Phosphoserine.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 99 99 Phosphoserine; by PKA.
 MOD_RES 215 215 Phosphothreonine; by PDPK1 (By  
Keyword
 3D-structure; Acetylation; cAMP; cAMP-binding; Cell membrane; Complete proteome; Cytoplasm; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 404 AA 
Protein Sequence
MSHIQIPPGL TELLQGYTVE VLRQQPPDLV EFAVEYFTRL REARAPASVL PAATPRQSLG 60
HPPPEPGPDR VADAKGDSES EEDEDLEVPV PSRFNRRVSV CAETYNPDEE EEDTDPRVIH 120
PKTDEQRCRL QEACKDILLF KNLDQEQLSQ VLDAMFERIV KADEHVIDQG DDGDNFYVIE 180
RGTYDILVTK DNQTRSVGQY DNRGSFGELA LMYNTPRAAT IVATSEGSLW GLDRVTFRRI 240
IVKNNAKKRK MFESFIESVP LLKSLEVSER MKIVDVIGEK IYKDGERIIT QGEKADSFYI 300
IESGEVSILI RSRTKSNKDG GNQEVEIARC HKGQYFGELA LVTNKPRAAS AYAVGDVKCL 360
VMDVQAFERL LGPCMDIMKR NISHYEEQLV KMFGSSVDLG NLGQ 404 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; NAS:UniProtKB.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0034199; P:activation of protein kinase A activity; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0006833; P:water transport; TAS:Reactome. 
Interpro
 IPR002373; cAMP/cGMP_kin.
 IPR012198; cAMP_dep_PK_reg_su.
 IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF02197; RIIa 
SMART
 SM00100; cNMP
 SM00394; RIIa 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 
PRINTS
 PR00103; CAMPKINASE.