UniProt Accession

 RELE_ECOLI; P0C077; P07008 

Genbank Protein ID

 X02405; U00096; AP009048 

Genbank Nucleotide ID

 CAA26251.1; AAC74636.1; BAA15262.1 

Protein Name

 mRNA interferase RelE 

Protein Synonyms/Alias

 Endoribonuclease RelE; Toxin RelE 

Gene Name

 relE 

Gene Synonyms/Alias

 b1563; JW1555 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
29	VREQLKKKLVEVLES	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Toxic component of a toxin-antitoxin (TA) module. A sequence-specific, ribosome-dependent mRNA endoribonuclease that inhibits translation during amino acid starvation (the stringent response). Acts by cleaving mRNA with high codon specificity in the ribosomal A site between positions 2 and 3. The stop codon UAG is cleaved at a fast rate while UAA and UGA are cleaved with intermediate and slow rates. mRNA cleavage can also occur in the ribosomal E site after peptide release from peptidyl-tRNA in the P site as well as on free 30S subunits. Overexpression of RelE results in the inhibition of bacterial growth and a sharp decrease in colony-forming ability which is inhibited by the labile cognate antitoxin RelB. Overexpression also sharply increases persisters (cells that neither grow or die in presence of bactericidal agent and are largely responsible for high levels of biofilm tolerance to antimicrobials). Acts with RelB as a corepressor of relBE transcription. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Endonuclease; Hydrolase; Nuclease; Reference proteome; Repressor; RNA-binding; rRNA-binding; Stress response; Toxin; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 95 AA 

Protein Sequence

Gene Ontology

 GO:0004521; F:endoribonuclease activity; IDA:EcoCyc.
 GO:0043022; F:ribosome binding; IDA:EcoCyc.
 GO:0070181; F:SSU rRNA binding; IPI:EcoCyc.
 GO:0034198; P:cellular response to amino acid starvation; IEP:EcoCyc.
 GO:0006402; P:mRNA catabolic process; IDA:EcoCyc.
 GO:0017148; P:negative regulation of translation; IDA:EcoCyc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0046677; P:response to antibiotic; IMP:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR007712; RelE/ParE_toxin. 

Pfam

 PF05016; Plasmid_stabil 

SMART

  

PROSITE

  

PRINTS