CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016223
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fermitin family homolog 3 
Protein Synonyms/Alias
 Kindlin-3; MIG2-like protein; Unc-112-related protein 2 
Gene Name
 FERMT3 
Gene Synonyms/Alias
 KIND3; MIG2B; URP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MAGMKTASGDYIubiquitination[1]
155KEKKKKEKEPEEELYubiquitination[1]
166EELYDLSKVVLAGGVubiquitination[1]
222RPSSLSDKTQLHSRWubiquitination[1, 2]
242CLMQQGIKAGDALWLubiquitination[1]
252DALWLRFKYYSFFDLubiquitination[1]
353LTTIPELKDHLRIFRubiquitination[1, 2, 3]
389ETTLSYYKSQDEAPGubiquitination[1]
405PIQQLNLKGCEVVPDubiquitination[1]
419DVNVSGQKFCIKLLVacetylation[3]
419DVNVSGQKFCIKLLVubiquitination[1, 3]
567VRFKGSRKDEILGIAubiquitination[1]
590LAVGDVVKTWRFSNMubiquitination[1, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs) (By similarity). 
Sequence Annotation
 DOMAIN 229 558 FERM.
 DOMAIN 354 457 PH.
 MOD_RES 11 11 Phosphotyrosine.
 MOD_RES 504 504 Phosphotyrosine.  
Keyword
 3D-structure; Alternative splicing; Cell adhesion; Cell junction; Cell projection; Complete proteome; Direct protein sequencing; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 667 AA 
Protein Sequence
MAGMKTASGD YIDSSWELRV FVGEEDPEAE SVTLRVTGES HIGGVLLKIV EQINRKQDWS 60
DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV ILRLPNRRAL RLRASFSQPL 120
FQAVAAICRL LSIRHPEELS LLRAPEKKEK KKKEKEPEEE LYDLSKVVLA GGVAPALFRG 180
MPAHFSDSAQ TEACYHMLSR PQPPPDPLLL QRLPRPSSLS DKTQLHSRWL DSSRCLMQQG 240
IKAGDALWLR FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL LEEIDCTEEE MMVFAALQYH 300
INKLSQSGEV GEPAGTDPGL DDLDVALSNL EVKLEGSAPT DVLDSLTTIP ELKDHLRIFR 360
IPRRPRKLTL KGYRQHWVVF KETTLSYYKS QDEAPGDPIQ QLNLKGCEVV PDVNVSGQKF 420
CIKLLVPSPE GMSEIYLRCQ DEQQYARWMA GCRLASKGRT MADSSYTSEV QAILAFLSLQ 480
RTGSGGPGNH PHGPDASAEG LNPYGLVAPR FQRKFKAKQL TPRILEAHQN VAQLSLAEAQ 540
LRFIQAWQSL PDFGISYVMV RFKGSRKDEI LGIANNRLIR IDLAVGDVVK TWRFSNMRQW 600
NVNWDIRQVA IEFDEHINVA FSCVSASCRI VHEYIGGYIF LSTRERARGE ELDEDLFLQL 660
TGGHEAF 667 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0002102; C:podosome; ISS:UniProtKB.
 GO:0005178; F:integrin binding; ISS:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0033622; P:integrin activation; IMP:UniProtKB.
 GO:0007159; P:leukocyte cell-cell adhesion; IMP:UniProtKB.
 GO:0070527; P:platelet aggregation; ISS:UniProtKB.
 GO:0033632; P:regulation of cell-cell adhesion mediated by integrin; IMP:UniProtKB. 
Interpro
 IPR019749; Band_41_domain.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF00373; FERM_M
 PF00169; PH 
SMART
 SM00295; B41
 SM00233; PH 
PROSITE
 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3
 PS50003; PH_DOMAIN 
PRINTS