CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022145
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin repeat and EF-hand domain-containing protein 1 
Protein Synonyms/Alias
 Ankyrin repeat domain-containing protein 5 
Gene Name
 ANKEF1 
Gene Synonyms/Alias
 ANKRD5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15LENLQIYKVLQCVRNubiquitination[1]
104MEILAKAKADMTIVDubiquitination[1, 2]
173VCLTFLEKGANPNAIubiquitination[1]
278AQRGCDLKWKNLDHKubiquitination[1]
509AGDLASLKKAFESGIubiquitination[2]
510GDLASLKKAFESGIPubiquitination[1]
521SGIPVDMKDNYYKTPubiquitination[2]
608SCRLDTVKYLLDIGAubiquitination[1, 2]
761DFMMPFQKNITEKARubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 REPEAT 47 76 ANK 1.
 REPEAT 184 213 ANK 2.
 REPEAT 217 246 ANK 3.
 REPEAT 250 279 ANK 4.
 DOMAIN 335 369 EF-hand.
 REPEAT 524 553 ANK 5.
 REPEAT 557 586 ANK 6.
 REPEAT 590 619 ANK 7.
 REPEAT 623 652 ANK 8.  
Keyword
 ANK repeat; Complete proteome; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 776 AA 
Protein Sequence
MALADKRLEN LQIYKVLQCV RNKDKKQIEK LTKLGYPELI NYTEPINGLS ALHLASVSND 60
IDMVSFLLDL GAHPDVQDRM GCTPTMRAAE LGHELSMEIL AKAKADMTIV DNEGKGVLFY 120
CILPTKRHYR CALIALEHGA DVNNSTYEGK PIFLRACEDA HDVKDVCLTF LEKGANPNAI 180
NSSTGRTALM EASREGVVEI VRGILERGGE VNAFDNDRHH AAHFAAKGGF FDILKLLFAY 240
NGDVGLISIN GNTPLHYAAM GGFADCCKYI AQRGCDLKWK NLDHKTPRAV AKEGGFKAAS 300
KEIRRAERIA NKLARPGAKN PNPLWALRLH DWSVEREAFL REAFAVLDRG DGSISKNDFV 360
MVLEERQDYA SSEQLAAIAH LHEKTRGGGV NINEFFKGTR YLNKSFVLGS YGPKKKEKGM 420
GKKGKKGKFV LPLPICVIPE YAFPRRQDGG PPYYMIETYK NVTDSSRFNR DHPPEHPIQD 480
DSVWYIDDSE KVFSNINIIT KAGDLASLKK AFESGIPVDM KDNYYKTPLM TACASGNIDV 540
VKFLLEKGAN VNATDNFLWT PLHFACHAGQ QDIVELLVES GALIDAASIN NSTPLNRAIE 600
SCRLDTVKYL LDIGAKFQLE NRKGHSAMDV AKAYADYRII DLIKEKLDNL PKPAENQKLK 660
GKTPPILKTE GPEIKKEEEL LSSIYGVPTT SEGKKVQKGN VVHLNSLITS GYTKKVDITF 720
IPRRIWSPEA TTAELIRKRE LRRERFTHEV DFDDFMMPFQ KNITEKARAL EAALKT 776 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 
Pfam
 PF12796; Ank_2 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50222; EF_HAND_2 
PRINTS
 PR01415; ANKYRIN.