CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007298
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nicotinamide N-methyltransferase 
Protein Synonyms/Alias
  
Gene Name
 NNMT 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MESGFTSKDTYLSHFmethylation[1]
8MESGFTSKDTYLSHFubiquitination[2]
23NPRDYLEKYYKFGSRubiquitination[2]
39SAESQILKHLLKNLFacetylation[3, 4]
43QILKHLLKNLFKIFCacetylation[4]
43QILKHLLKNLFKIFCubiquitination[2]
47HLLKNLFKIFCLDGVubiquitination[2]
210YYMIGEQKFSSLPLGubiquitination[2]
Reference
 [1] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
 Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. 
Sequence Annotation
 REGION 63 64 S-adenosyl-L-methionine binding.
 REGION 142 143 S-adenosyl-L-methionine binding.
 BINDING 20 20 S-adenosyl-L-methionine.
 BINDING 25 25 S-adenosyl-L-methionine.
 BINDING 69 69 S-adenosyl-L-methionine.
 BINDING 85 85 S-adenosyl-L-methionine.
 BINDING 90 90 S-adenosyl-L-methionine.
 BINDING 163 163 S-adenosyl-L-methionine.
 MOD_RES 39 39 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 264 AA 
Protein Sequence
MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI 60
DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN 120
RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL 180
RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ 240
SYSSTMANNE GLFSLVARKL SRPL 264 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0008112; F:nicotinamide N-methyltransferase activity; TAS:ProtInc.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0010243; P:response to organic nitrogen; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 
Interpro
 IPR025818; NNMT.
 IPR025820; NNMT/PNMT/TEMT_CS.
 IPR000940; NNMT_TEMT_trans. 
Pfam
 PF01234; NNMT_PNMT_TEMT 
SMART
  
PROSITE
 PS01100; NNMT_PNMT_TEMT 
PRINTS