CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012744
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase 
Gene Name
 HADH 
Gene Synonyms/Alias
 HAD; HADHSC; SCHAD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87KKFAENLKAGDEFVEubiquitination[1]
179FNPVPVMKLVEVIKTacetylation[2]
185MKLVEVIKTPMTSQKacetylation[2]
202ESLVDFSKALGKHPVacetylation[2]
202ESLVDFSKALGKHPVubiquitination[3]
241YERGDASKEDIDTAMacetylation[2, 4]
312KTGEGFYKYK*****acetylation[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Plays an essential role in the mitochondrial beta- oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. 
Sequence Annotation
 NP_BIND 34 39 NAD.
 BINDING 57 57 NAD.
 BINDING 73 73 Coenzyme A.
 BINDING 80 80 Coenzyme A.
 BINDING 122 122 NAD.
 BINDING 127 127 NAD.
 BINDING 149 149 Coenzyme A.
 BINDING 149 149 NAD.
 BINDING 173 173 NAD.
 BINDING 305 305 NAD.
 MOD_RES 185 185 N6-acetyllysine.
 MOD_RES 202 202 N6-acetyllysine.
 MOD_RES 241 241 N6-acetyllysine.
 MOD_RES 312 312 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Disease mutation; Fatty acid metabolism; Lipid metabolism; Mitochondrion; NAD; Oxidoreductase; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 314 AA 
Protein Sequence
MAFVTRQFMR SVSSSSTASA SAKKIIVKHV TVIGGGLMGA GIAQVAAATG HTVVLVDQTE 60
DILAKSKKGI EESLRKVAKK KFAENLKAGD EFVEKTLSTI ATSTDAASVV HSTDLVVEAI 120
VENLKVKNEL FKRLDKFAAE HTIFASNTSS LQITSIANAT TRQDRFAGLH FFNPVPVMKL 180
VEVIKTPMTS QKTFESLVDF SKALGKHPVS CKDTPGFIVN RLLVPYLMEA IRLYERGDAS 240
KEDIDTAMKL GAGYPMGPFE LLDYVGLDTT KFIVDGWHEM DAENPLHQPS PSLNKLVAEN 300
KFGKKTGEGF YKYK 314 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; TAS:Reactome.
 GO:0070403; F:NAD+ binding; IEA:InterPro.
 GO:0006635; P:fatty acid beta-oxidation; TAS:Reactome. 
Interpro
 IPR022694; 3-OHacyl-CoA_DH.
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00725; 3HCDH
 PF02737; 3HCDH_N 
SMART
  
PROSITE
 PS00067; 3HCDH 
PRINTS