CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020223
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 THUMP domain-containing protein 3 
Protein Synonyms/Alias
  
Gene Name
 THUMPD3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
237TCNRAGEKHCFTSNEubiquitination[1]
258GAVQDYFKWKADMTNubiquitination[1]
369NIASLLTKSQIKEGKubiquitination[1, 2, 3, 4]
373LLTKSQIKEGKPSWGubiquitination[1]
376KSQIKEGKPSWGLPIubiquitination[1]
411VTDLPFGKRMGSKKRubiquitination[1]
449VLLTQDTKCFTKALSubiquitination[1]
464GMRHVWRKVDTVWVNubiquitination[1]
506RGTLWQCKE******ubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 DOMAIN 165 285 THUMP.  
Keyword
 Complete proteome; Methyltransferase; Polymorphism; Reference proteome; RNA-binding; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MCDIEEATNQ LLDVNLHENQ KSVQVTESDL GSESELLVTI GATVPTGFEQ TAADEVREKL 60
GSSCKISRDR GKIYFVISVE SLAQVHCLRS VDNLFVVVQE FQDYQFKQTK EEVLKDFEDL 120
AGKLPWSNPL KVWKINASFK KKKAKRKKIN QNSSKEKINN GQEVKIDQRN VKKEFTSHAL 180
DSHILDYYEN PAIKEDVSTL IGDDLASCKD ETDESSKEET EPQVLKFRVT CNRAGEKHCF 240
TSNEAARDFG GAVQDYFKWK ADMTNFDVEV LLNIHDNEVI VGIALTEESL HRRNITHFGP 300
TTLRSTLAYG MLRLCDPLPY DIIVDPMCGT GAIPIEGATE WSDCFHIAGD NNPLAVNRAA 360
NNIASLLTKS QIKEGKPSWG LPIDAVQWDI CNLPLRTGSV DIIVTDLPFG KRMGSKKRNW 420
NLYPACLREM SRVCTPTTGR AVLLTQDTKC FTKALSGMRH VWRKVDTVWV NVGGLRAAVY 480
VLIRTPQAFV HPSEQDGERG TLWQCKE 507 
Gene Ontology
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR000241; RNA_methylase_dom.
 IPR004114; THUMP. 
Pfam
 PF02926; THUMP
 PF01170; UPF0020 
SMART
 SM00981; THUMP 
PROSITE
 PS51165; THUMP
 PS01261; UPF0020 
PRINTS