UniProt Accession

 PUR1_RAT; P35433 

Genbank Protein ID

 D10853; BC086999; D37978 

Genbank Nucleotide ID

 BAA01626.1; AAH86999.1; BAA21036.1 

Protein Name

 Amidophosphoribosyltransferase 

Protein Synonyms/Alias

 ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT 

Gene Name

 Ppat 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
60	SDGSSVPKFRVHKGM	acetylation	[1]
80	VFTEDNLKKLYVSNL	acetylation	[1]
81	FTEDNLKKLYVSNLG	acetylation	[1]
371	LRQLGVAKKFGVLSD	acetylation	[1]
372	RQLGVAKKFGVLSDN	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

  

Sequence Annotation

 DOMAIN 12 261 Glutamine amidotransferase type-2.
 ACT_SITE 12 12 For GATase activity (By similarity).
 METAL 280 280 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 327 327 Magnesium (By similarity).
 METAL 389 389 Magnesium (By similarity).
 METAL 390 390 Magnesium (By similarity).
 METAL 426 426 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 503 503 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 506 506 Iron-sulfur (4Fe-4S) (By similarity).  

Keyword

 4Fe-4S; Allosteric enzyme; Complete proteome; Direct protein sequencing; Glutamine amidotransferase; Glycosyltransferase; Iron; Iron-sulfur; Magnesium; Metal-binding; Purine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 517 AA 

Protein Sequence

Gene Ontology

 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0004044; F:amidophosphoribosyltransferase activity; IDA:RGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0035690; P:cellular response to drug; IEP:RGD.
 GO:0032869; P:cellular response to insulin stimulus; IEP:RGD.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEP:RGD.
 GO:0006543; P:glutamine catabolic process; IDA:RGD.
 GO:0001822; P:kidney development; IEP:RGD.
 GO:0007595; P:lactation; IEP:RGD.
 GO:0060135; P:maternal process involved in female pregnancy; IEP:RGD.
 GO:0009116; P:nucleoside metabolic process; IEA:InterPro.
 GO:0031100; P:organ regeneration; IEP:RGD.
 GO:0051289; P:protein homotetramerization; IDA:RGD.
 GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
 GO:0009168; P:purine ribonucleoside monophosphate biosynthetic process; IDA:RGD.
 GO:0019693; P:ribose phosphate metabolic process; IDA:RGD. 

Interpro

 IPR005854; Amd_phspho_trans.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR000836; PRibTrfase_dom. 

Pfam

 PF00310; GATase_2
 PF00156; Pribosyltran 

SMART

  

PROSITE

 PS51278; GATASE_TYPE_2
 PS00103; PUR_PYR_PR_TRANSFER 

PRINTS