UniProt Accession

 ATG3_MOUSE; Q9CPX6; Q3TXJ9 

Genbank Protein ID

 AB079386; AK005266; AK011409; AK078877; AK150914; AK159231; BC010809 

Genbank Nucleotide ID

 BAC57452.1; BAB23918.1; BAB27600.1; BAC37437.1; BAE29952.1; BAE34917.1; AAH10809.1 

Protein Name

 Ubiquitin-like-conjugating enzyme ATG3 

Protein Synonyms/Alias

 Autophagy-related protein 3; APG3-like 

Gene Name

 Atg3 

Gene Synonyms/Alias

 Apg3l 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
11	VINTVKGKALEVAEY	ubiquitination	[1]
24	EYLTPVLKESKFKET	ubiquitination	[1]
77	DKQFLVTKNVPCYKR	acetylation	[2]
131	KEITLESKDSIKLQD	ubiquitination	[1]
208	DLYITYDKYYQTPRL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1- E2 complex with ATG3. 

Sequence Annotation

 ACT_SITE 264 264 Glycyl thioester intermediate (Probable).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 CROSSLNK 243 243 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Autophagy; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Protein transport; Reference proteome; Transport; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0000153; C:cytoplasmic ubiquitin ligase complex; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0019777; F:Atg12 ligase activity; IDA:UniProtKB.
 GO:0019776; F:Atg8 ligase activity; IMP:UniProtKB.
 GO:0000045; P:autophagic vacuole assembly; IMP:UniProtKB.
 GO:0043653; P:mitochondrial fragmentation involved in apoptotic process; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0016567; P:protein ubiquitination; IEA:Compara. 

Interpro

 IPR007135; Autophagy-rel_prot_3.
 IPR019461; Autophagy-rel_prot_3_C.
 IPR007134; Autophagy-rel_prot_3_N. 

Pfam

 PF03987; Autophagy_act_C
 PF10381; Autophagy_Cterm
 PF03986; Autophagy_N 

SMART

  

PROSITE

  

PRINTS