CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014257
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Krueppel-like factor 4 
Protein Synonyms/Alias
 Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor 
Gene Name
 Klf4 
Gene Synonyms/Alias
 Ezf; Gklf; Zie 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
225PGGGLMGKFVLKASLacetylation[1]
229LMGKFVLKASLTTPGacetylation[1]
275PRMCPKIKQEAVPSCsumoylation[2]
Reference
 [1] Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation.
 Evans PM, Zhang W, Chen X, Yang J, Bhakat KK, Liu C.
 J Biol Chem. 2007 Nov 23;282(47):33994-4002. [PMID: 17908689]
 [2] A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.
 Du JX, McConnell BB, Yang VW.
 J Biol Chem. 2010 Sep 3;285(36):28298-308. [PMID: 20584900] 
Functional Description
 Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity). 
Sequence Annotation
 ZN_FING 400 424 C2H2-type 1.
 ZN_FING 430 454 C2H2-type 2.
 ZN_FING 460 482 C2H2-type 3.
 REGION 443 474 Interaction with target DNA.
 MOD_RES 251 251 Phosphoserine.
 CROSSLNK 32 32 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Activator; Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 483 AA 
Protein Sequence
MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRPAGAP TNRWREELSH MKRLPPLPGR 60
PYDLAATVAT DLESGGAGAA CSSNNPALLA RRETEEFNDL LDLDFILSNS LTHQESVAAT 120
VTTSASASSS SSPASSGPAS APSTCSFSYP IRAGGDPGVA ASNTGGGLLY SRESAPPPTA 180
PFNLADINDV SPSGGFVAEL LRPELDPVYI PPQQPQPPGG GLMGKFVLKA SLTTPGSEYS 240
SPSVISVSKG SPDGSHPVVV APYSGGPPRM CPKIKQEAVP SCTVSRSLEA HLSAGPQLSN 300
GHRPNTHDFP LGRQLPTRTT PTLSPEELLN SRDCHPGLPL PPGFHPHPGP NYPPFLPDQM 360
QSQVPSLHYQ ELMPPGSCLP EEPKPKRGRR SWPRKRTATH TCDYAGCGKT YTKSSHLKAH 420
LRTHTGEKPY HCDWDGCGWK FARSDELTRH YRKHTGHRPF QCQKCDRAFS RSDHLALHMK 480
RHF 483 
Gene Ontology
 GO:0000790; C:nuclear chromatin; IEA:Compara.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IEA:Compara.
 GO:0003690; F:double-stranded DNA binding; IEA:Compara.
 GO:0035014; F:phosphatidylinositol 3-kinase regulator activity; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
 GO:0001010; F:sequence-specific DNA binding transcription factor recruiting transcription factor activity; IDA:BHF-UCL.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0071363; P:cellular response to growth factor stimulus; IEA:Compara.
 GO:0009913; P:epidermal cell differentiation; IMP:MGI.
 GO:0048730; P:epidermis morphogenesis; IMP:MGI.
 GO:0045444; P:fat cell differentiation; IMP:BHF-UCL.
 GO:0090051; P:negative regulation of cell migration involved in sprouting angiogenesis; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IDA:MGI.
 GO:2000342; P:negative regulation of chemokine (C-X-C motif) ligand 2 production; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IEA:Compara.
 GO:0034115; P:negative regulation of heterotypic cell-cell adhesion; IEA:Compara.
 GO:0045415; P:negative regulation of interleukin-8 biosynthetic process; IEA:Compara.
 GO:0014740; P:negative regulation of muscle hyperplasia; IEA:Compara.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IEA:Compara.
 GO:0014067; P:negative regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0051898; P:negative regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0060761; P:negative regulation of response to cytokine stimulus; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0046985; P:positive regulation of hemoglobin biosynthetic process; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0031077; P:post-embryonic camera-type eye development; IMP:MGI.
 GO:0035166; P:post-embryonic hemopoiesis; IEA:Compara.
 GO:0032526; P:response to retinoic acid; IDA:MGI.
 GO:0019827; P:stem cell maintenance; IDA:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; IDA:BHF-UCL. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS