CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024577
UniProt Accession
Genbank Protein ID
 Z84812 
Genbank Nucleotide ID
  
Protein Name
 Putative WAS protein family homolog 4 
Protein Synonyms/Alias
 Protein FAM39CP 
Gene Name
 WASH4P 
Gene Synonyms/Alias
 FAM39CP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
81QVQAIGEKVSLAQAKubiquitination[1]
88KVSLAQAKIEKIKGSubiquitination[1]
151DERALQEKLKDFPVCubiquitination[1]
198TTENLYKKYVFLDPLubiquitination[1]
233DAPLSISKREQLEQQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (By similarity). 
Sequence Annotation
 DOMAIN 374 396 WH2.
 REGION 1 180 WHD1.  
Keyword
 Actin-binding; Complete proteome; Endosome; Membrane; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 477 AA 
Protein Sequence
MSGVMCLKAS DTWASGIRSQ PQGCLGKWRS MRCKHTRMHL AHLGNSRQLI SLGPPRTRED 60
GSRISQQVEQ SRSQVQAIGE KVSLAQAKIE KIKGSKKAIK VFSSAKYPAP ERLQEYGSIF 120
TDAQDPGLQR RPRHRIQSKQ RPLDERALQE KLKDFPVCVS TKPEPEDDAE EGLGGLPSNI 180
SSVSSLLLFN TTENLYKKYV FLDPLAGAVT KTHVMLGAET EEKLFDAPLS ISKREQLEQQ 240
VPENYFYVPD LGQVPEIDVP SYLPDLPGIA NDLMYIADLG PGIAPSAPGT IPELPTFHTE 300
VAEPLKVDLQ DGVLTPPPPP PPPPPAPEVL ASAPPLPPST AAPVGQGARQ DDSSSSASPS 360
VQGAPREVVD PSGGWATLLE SIRQAGGIGK AKLRSMKERK LEKQQQKEQE QVRATSQGGH 420
LMSDLFNKLV MRRKGISGKG PGAGDGPGGA FARVSDSIPP LPPPQQPQAE DEDDWES 477 
Gene Ontology
 GO:0005769; C:early endosome; ISS:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; ISS:UniProtKB.
 GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
 GO:0071203; C:WASH complex; ISS:UniProtKB.
 GO:0043014; F:alpha-tubulin binding; ISS:UniProtKB.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
 GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB. 
Interpro
 IPR021854; WASH_WASD. 
Pfam
 PF11945; WASH_WAHD 
SMART
  
PROSITE
 PS51082; WH2 
PRINTS