CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006158
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin cytoskeleton-regulatory complex protein PAN1 
Protein Synonyms/Alias
 Mitochondrial distribution of proteins protein 3 
Gene Name
 PAN1 
Gene Synonyms/Alias
 DIM2; MDP3; MIP3; YIR006C; YIB6C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
719KIDALSYKNNDDDVLubiquitination[1]
752SFSSPSAKSVNHSSSubiquitination[1]
920RIQQENGKNQEIIKDubiquitination[1]
1010SPSYSQFKTAEERAAubiquitination[1]
1295NRVAVDNKVEEAKIGubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization. Required for the bipolar budding of diploid cells and the correct distribution of chitin at the cell surface. 
Sequence Annotation
 REPEAT 142 153 1-1.
 REPEAT 164 175 1-2.
 REPEAT 188 199 1-3.
 REPEAT 215 226 1-4.
 REPEAT 235 246 1-5.
 DOMAIN 270 359 EH 1.
 REPEAT 328 350 2-1.
 REPEAT 392 403 1-6.
 REPEAT 409 420 1-7.
 REPEAT 422 433 1-8.
 REPEAT 446 457 1-9.
 REPEAT 467 478 1-10.
 REPEAT 498 509 1-11.
 REPEAT 510 518 1-12.
 REPEAT 538 548 1-13.
 REPEAT 549 556 1-14.
 REPEAT 564 575 1-15.
 DOMAIN 600 689 EH 2.
 REPEAT 658 680 2-2.
 REPEAT 1084 1089 3-1.
 REPEAT 1090 1095 3-2.
 REPEAT 1096 1101 3-3.
 REPEAT 1102 1107 3-4.
 REPEAT 1108 1113 3-5.
 REPEAT 1114 1119 3-6.
 REPEAT 1120 1125 3-7.
 REPEAT 1315 1320 4-1.
 REPEAT 1321 1326 4-2.
 REPEAT 1327 1332 4-3.
 REPEAT 1340 1345 4-4.
 REPEAT 1346 1350 4-5.
 REPEAT 1355 1360 4-6.
 REPEAT 1361 1366 4-7.
 REPEAT 1372 1377 4-8.
 REGION 142 575 15 X 12 AA tandem repeats of [SPNAG]-
 REGION 328 680 2 X 23 AA repeats of F-A-L-[AG]-M-H-L-
 REGION 1084 1125 7 X 6 AA tandem repeats of Q-[PS]-T-Q-P-
 REGION 1315 1377 8 X 6 AA repeats of [ATVSP]-P-[LVI]-P-
 MOD_RES 241 241 Phosphothreonine.
 MOD_RES 570 570 Phosphothreonine.
 MOD_RES 747 747 Phosphoserine.
 MOD_RES 757 757 Phosphoserine.
 MOD_RES 993 993 Phosphothreonine.
 MOD_RES 995 995 Phosphothreonine.
 MOD_RES 1003 1003 Phosphoserine.
 MOD_RES 1180 1180 Phosphoserine.
 MOD_RES 1250 1250 Phosphoserine.
 MOD_RES 1253 1253 Phosphoserine.
 MOD_RES 1281 1281 Phosphoserine.
 MOD_RES 1321 1321 Phosphothreonine.  
Keyword
 Actin-binding; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Endocytosis; Endosome; Membrane; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1480 AA 
Protein Sequence
MYNPYQQQGM GYQQQQQQQQ QQPNGFYPQQ QQGQSSNQPQ GQPQPQQQMA FNQPQATGIG 60
GMPQSFGNSF SSMPQQPQTG YNNNGNNGSV YGNGNFGQQP QQQQQQAKPQ HTGYVPNSSM 120
PMMNTTGTMP PPNPAQQPQL QSIQPQGTGY YQAANTANVH SVQPLQSQGT GYYVSTPNLI 180
SSNQTQQPLQ AQGTGYYQSQ PQQVPPPQQA QSLQPLKPQQ TGFYLQPQNQ APLEPLKPTA 240
TGFVNSFANN GLNNDIKIPA IRLSFITAQD QAKFETLFRS IVTNGSNTVS GANCRKILMR 300
SGLPPSQLAR IWTLCDTSKA GELLFPEFAL AMHLINDVLQ GDTIPYELDS KTKNEVSSFI 360
DAINLSIANQ DSSANDAPKT PFDEFITAGV QNLQPQPTGY MPQTSFGIPL QSQITGGGVA 420
SALNPQSTGF MAPTTFNMSM NTGTPGLNPQ ITGGAPASMQ PNITGNALQP QTTGMMPQTT 480
GMMPQTTGMM PQTSFGVNLG PQLTGGALQS QYTGGYGSVM PQQSGPASMP NLSFNQQGLQ 540
SQLTGLQPQP TGFLPPSNFS ATMPLTAQKT GFGNNEIYTK SNFNNNLIDN SSQDKISTEE 600
KSLFYKIFET FDTQNKGLLD SPTAVEIFRK SGLNRADLEQ IWNLCDINNT GQLNKQEFAL 660
GMHLVYGKLN GKPIPNVLPS SLIPSSTKLL DNLKNQLKTE PTTTKEKPSF GKIDALSYKN 720
NDDDVLPNYR NRRKVYSAKN EEQSSFSSPS AKSVNHSSST LQTDDISVDK TVEKKTAKPK 780
YAGFSREINL KNIASLENEI KNISNPENCY DSSIPSDLTS RFDAIIAKLP NLFNEISTID 840
NEITNAKIQL YRKKNPSSII GSGPNGEITE NDRKKAKSRA LLRARMSALT GKSTESEDSL 900
SMEDEQQSAE IKRIQQENGK NQEIIKDIRS SISDISASLK STMTGSNMIS NQEFERWEFG 960
IGLEDGVREF LDDLKSNSNK SVTESSPFVP SSTPTPVDDR SSSPSYSQFK TAEERAAYLK 1020
EQAKKRMKEK LAKFDKNRRN VTQSSRSISS ENSREQPQQI AGSSNLVEPR ATPFQEEKYV 1080
EVAQPTQPVQ STQPVQPTQP VQPTQPVQPT QPVQPTQPVQ PTQPVQNVYN AKQESDDEDE 1140
DDEEKRLQEE LKRLKLKKKA DKEKRLAALR KQIEDAQNES DEEETNGKDN FGGHVNVPQA 1200
APVAPSAAFS QNSTNAPRSV HAAVTPAAGK NSTGLPSTTM GHNPYFKDAS ASSTSTFDAR 1260
AAEMQRRIQR GLDEDEDDGW SDEDESNNRV AVDNKVEEAK IGHPDHARAP PVTAAPLPSV 1320
TPVPPAVPVP QANTSNEKSS PIPIAPIPPS VTQEPPVPLA PPLPAVDGFQ EPPIPSAPAI 1380
ATAVQKSGSS TPALAGGVLP PPPPLPTQQA STSEPIIAHV DNYNGAEKGT GAYGSDSDDD 1440
VLSIPESVGT DEEEEGAQPV STAGIPSIPP AGIPPPPPLP 1480 
Gene Ontology
 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0000147; P:actin cortical patch assembly; IMP:SGD.
 GO:0007120; P:axial cellular bud site selection; IMP:SGD.
 GO:0007121; P:bipolar cellular bud site selection; IMP:SGD.
 GO:0006897; P:endocytosis; IMP:SGD. 
Interpro
 IPR013182; DUF1720.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR026812; Pan1. 
Pfam
 PF08226; DUF1720 
SMART
 SM00054; EFh
 SM00027; EH 
PROSITE
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS