UniProt Accession

 NKAP_HUMAN; Q8N5F7; Q6IPW6; Q96BQ2; Q9H638 

Genbank Protein ID

 AY388958; AK026279; BC015354; BC032442; BC071686 

Genbank Nucleotide ID

 AAQ90402.1; BAB15428.1; AAH15354.1; AAH32442.1; AAH71686.1 

Protein Name

 NF-kappa-B-activating protein 

Protein Synonyms/Alias

  

Gene Name

 NKAP 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
112	SRPYGSDKPWPSLLD	acetylation	[1]
283	NPWKDRTKAEEPSDL	ubiquitination	[2, 3]
326	AAMAEYVKAGKRIPR	ubiquitination	[3]
379	QIYSADEKRALASFN	ubiquitination	[2, 3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter. 

Sequence Annotation

 REGION 179 272 Necessary for interaction with CIR1.
 REGION 273 415 Necessary for interaction with HDAC3 and
 MOD_RES 112 112 N6-acetyllysine.
 MOD_RES 149 149 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Notch signaling pathway; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 415 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0031490; F:chromatin DNA binding; IEA:Compara.
 GO:0030851; P:granulocyte differentiation; IEA:Compara.
 GO:0071425; P:hematopoietic stem cell proliferation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
 GO:0046638; P:positive regulation of alpha-beta T cell differentiation; IMP:UniProtKB.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0033077; P:T cell differentiation in thymus; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR009269; DUF926. 

Pfam

 PF06047; SynMuv_product 

SMART

  

PROSITE

  

PRINTS