| Tag | Content |
|---|
| CPLM ID | CPLM-002356 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Bifunctional protein BirA |
Protein Synonyms/Alias | Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase |
Gene Name | birA |
Gene Synonyms/Alias | bioR; dhbB; b3973; JW3941 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 172 | GADKVRVKWPNDLYL | acetylation | [1] | | 267 | PYLSRWEKLDNFINR | acetylation | [1] | | 277 | NFINRPVKLIIGDKE | acetylation | [1] | | 283 | VKLIIGDKEIFGISR | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting proteins. |
Sequence Annotation | DNA_BIND 22 41 H-T-H motif. |
Keyword | 3D-structure; ATP-binding; Biotin; Complete proteome; DNA-binding; Ligase; Nucleotide-binding; Reference proteome; Repressor; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 321 AA |
Protein Sequence | MKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL 60
PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG 120
RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ 180
DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA 240
MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL 300
EQDGIIKPWM GGEISLRSAE K 321 |
Gene Ontology | GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004077; F:biotin-[acetyl-CoA-carboxylase] ligase activity; IDA:EcoliWiki. GO:0003677; F:DNA binding; IDA:EcoliWiki. GO:0009102; P:biotin biosynthetic process; IMP:EcoliWiki. GO:0006464; P:cellular protein modification process; IEA:InterPro. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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