CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007500
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Metabotropic glutamate receptor 5 
Protein Synonyms/Alias
 mGluR5 
Gene Name
 GRM5 
Gene Synonyms/Alias
 GPRC1E; MGLUR5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
259AGEQSFDKLLKKLTSubiquitination[1, 2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system and generates a calcium-activated chloride current. 
Sequence Annotation
 CARBOHYD 88 88 N-linked (GlcNAc...) (Potential).
 CARBOHYD 210 210 N-linked (GlcNAc...) (Potential).
 CARBOHYD 378 378 N-linked (GlcNAc...) (Potential).
 CARBOHYD 382 382 N-linked (GlcNAc...) (Potential).
 CARBOHYD 445 445 N-linked (GlcNAc...) (Potential).
 CARBOHYD 734 734 N-linked (GlcNAc...) (Potential).
 DISULFID 57 99 By similarity.
 DISULFID 241 530 By similarity.
 DISULFID 365 381 By similarity.
 DISULFID 419 426 By similarity.
 DISULFID 511 531 By similarity.
 DISULFID 515 534 By similarity.
 DISULFID 537 549 By similarity.
 DISULFID 552 565 By similarity.  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1212 AA 
Protein Sequence
MVLLLILSVL LLKEDVRGSA QSSERRVVAH MPGDIIIGAL FSVHHQPTVD KVHERKCGAV 60
REQYGIQRVE AMLHTLERIN SDPTLLPNIT LGCEIRDSCW HSAVALEQSI EFIRDSLISS 120
EEEEGLVRCV DGSSSSFRSK KPIVGVIGPG SSSVAIQVQN LLQLFNIPQI AYSATSMDLS 180
DKTLFKYFMR VVPSDAQQAR AMVDIVKRYN WTYVSAVHTE GNYGESGMEA FKDMSAKEGI 240
CIAHSYKIYS NAGEQSFDKL LKKLTSHLPK ARVVACFCEG MTVRGLLMAM RRLGLAGEFL 300
LLGSDGWADR YDVTDGYQRE AVGGITIKLQ SPDVKWFDDY YLKLRPETNH RNPWFQEFWQ 360
HRFQCRLEGF PQENSKYNKT CNSSLTLKTH HVQDSKMGFV INAIYSMAYG LHNMQMSLCP 420
GYAGLCDAMK PIDGRKLLES LMKTNFTGVS GDTILFDENG DSPGRYEIMN FKEMGKDYFD 480
YINVGSWDNG ELKMDDDEVW SKKSNIIRSV CSEPCEKGQI KVIRKGEVSC CWTCTPCKEN 540
EYVFDEYTCK ACQLGSWPTD DLTGCDLIPV QYLRWGDPEP IAAVVFACLG LLATLFVTVV 600
FIIYRDTPVV KSSSRELCYI ILAGICLGYL CTFCLIAKPK QIYCYLQRIG IGLSPAMSYS 660
ALVTKTNRIA RILAGSKKKI CTKKPRFMSA CAQLVIAFIL ICIQLGIIVA LFIMEPPDIM 720
HDYPSIREVY LICNTTNLGV VTPLGYNGLL ILSCTFYAFK TRNVPANFNE AKYIAFTMYT 780
TCIIWLAFVP IYFGSNYKII TMCFSVSLSA TVALGCMFVP KVYIILAKPE RNVRSAFTTS 840
TVVRMHVGDG KSSSAASRSS SLVNLWKRRG SSGETLRYKD RRLAQHKSEI ECFTPKGSMG 900
NGGRATMSSS NGKSVTWAQN EKSSRGQHLW QRLSIHINKK ENPNQTAVIK PFPKSTESRG 960
LGAGAGAGGS AGGVGATGGA GCAGAGPGGP ESPDAGPKAL YDVAEAEEHF PAPARPRSPS 1020
PISTLSHRAG SASRTDDDVP SLHSEPVARS SSSQGSLMEQ ISSVVTRFTA NISELNSMML 1080
STAAPSPGVG APLCSSYLIP KEIQLPTTMT TFAEIQPLPA IEVTGGAQPA AGAQAAGDAA 1140
RESPAAGPEA AAAKPDLEEL VALTPPSPFR DSVDSGSTTP NSPVSESALC IPSSPKYDTL 1200
IIRDYTQSSS SL 1212 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0043198; C:dendritic shaft; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0014069; C:postsynaptic density; IEA:Compara.
 GO:0004930; F:G-protein coupled receptor activity; TAS:UniProtKB.
 GO:0008066; F:glutamate receptor activity; TAS:UniProtKB.
 GO:0000185; P:activation of MAPKKK activity; IEA:Compara.
 GO:0002029; P:desensitization of G-protein coupled receptor protein signaling pathway; IEA:Compara.
 GO:0007612; P:learning; IEA:Compara.
 GO:0007626; P:locomotory behavior; IEA:Compara.
 GO:0040013; P:negative regulation of locomotion; IEA:Compara.
 GO:0007206; P:phospholipase C-activating G-protein coupled glutamate receptor signaling pathway; TAS:ProtInc.
 GO:0048170; P:positive regulation of long-term neuronal synaptic plasticity; IEA:Compara.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0007268; P:synaptic transmission; TAS:ProtInc. 
Interpro
 IPR001828; ANF_lig-bd_rcpt.
 IPR000337; GPCR_3.
 IPR011500; GPCR_3_9-Cys_dom.
 IPR017978; GPCR_3_C.
 IPR017979; GPCR_3_CS.
 IPR000162; GPCR_3_mtglu_rcpt.
 IPR000202; GPCR_3_mtglu_rcpt_5.
 IPR019588; Metabotropic_Glu_rcpt_Homer-bd. 
Pfam
 PF00003; 7tm_3
 PF01094; ANF_receptor
 PF10606; GluR_Homer-bdg
 PF07562; NCD3G 
SMART
  
PROSITE
 PS00979; G_PROTEIN_RECEP_F3_1
 PS00980; G_PROTEIN_RECEP_F3_2
 PS00981; G_PROTEIN_RECEP_F3_3
 PS50259; G_PROTEIN_RECEP_F3_4 
PRINTS
 PR00248; GPCRMGR.
 PR01055; MTABOTROPC5R.
 PR00593; MTABOTROPICR.