CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015827
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heterogeneous nuclear ribonucleoprotein Q 
Protein Synonyms/Alias
 hnRNP Q; Glycine- and tyrosine-rich RNA-binding protein; GRY-RBP; NS1-associated protein 1; Synaptotagmin-binding, cytoplasmic RNA-interacting protein; pp68 
Gene Name
 Syncrip 
Gene Synonyms/Alias
 Hnrpq; Nsap1; Nsap1l 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
100AFLCGVMKTYRQREKubiquitination[1]
125GPDEAKIKALLERTGubiquitination[1]
168GTEIFVGKIPRDLFEubiquitination[1]
221EAAQEAVKLYNNHEIubiquitination[1]
336PDPEVMAKVKVLFVRubiquitination[1]
363KSFSQFGKLERVKKLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD- mediated complex that promotes MYC mRNA stability. Isoform 1 and isoform 2 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences (By similarity). Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself (By similarity). May be involved in translationally coupled mRNA turnover. Implicated with other RNA- binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding- region determinant of instability (mCRD) domain (By similarity). Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 2 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. 
Sequence Annotation
 DOMAIN 162 241 RRM 1.
 DOMAIN 243 325 RRM 2.
 DOMAIN 338 408 RRM 3.
 REPEAT 448 450 1-1.
 REPEAT 451 453 1-2.
 REPEAT 460 464 2-1.
 REPEAT 469 472 2-2.
 REPEAT 478 480 1-3.
 REPEAT 485 488 2-3.
 REPEAT 498 500 1-4.
 REPEAT 526 528 1-5.
 REPEAT 539 541 1-6.
 REPEAT 554 556 1-7.
 REPEAT 557 559 1-8.
 REGION 400 561 Interaction with APOBEC1 (By similarity).
 REGION 448 559 8 X 3 AA repeats of R-G-G.
 REGION 460 488 3 X 4 AA repeats of Y-Y-G-Y.
 REGION 518 549 Interaction with SMN (By similarity).
 MOTIF 564 578 Bipartite nuclear localization signal
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 221 221 N6-acetyllysine (By similarity).
 MOD_RES 363 363 N6-acetyllysine (By similarity).
 MOD_RES 373 373 Phosphotyrosine (By similarity).
 MOD_RES 444 444 Asymmetric dimethylarginine; by PRMT1;
 MOD_RES 444 444 Omega-N-methylarginine; by PRMT1;
 MOD_RES 496 496 Omega-N-methylarginine; by PRMT1 (By
 MOD_RES 510 510 Asymmetric dimethylarginine; by PRMT1 (By
 MOD_RES 518 518 Asymmetric dimethylarginine; by PRMT1;
 MOD_RES 518 518 Omega-N-methylarginine; by PRMT1;
 MOD_RES 536 536 Asymmetric dimethylarginine; by PRMT1;
 MOD_RES 536 536 Omega-N-methylarginine; by PRMT1;
 MOD_RES 539 539 Asymmetric dimethylarginine; by PRMT1;
 MOD_RES 539 539 Omega-N-methylarginine; by PRMT1;
 MOD_RES 587 587 Phosphoserine (By similarity).
 CROSSLNK 123 123 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 623 AA 
Protein Sequence
MATEHVNGNG TEEPMDTTSA VIHSENFQTL LDAGLPQKVA EKLDEIYVAG LVAHSDLDER 60
AIEALKEFNE DGALAVLQQF KDSDLSHVQN KSAFLCGVMK TYRQREKQGT KVADSSKGPD 120
EAKIKALLER TGYTLDVTTG QRKYGGPPPD SVYSGQQPSV GTEIFVGKIP RDLFEDELVP 180
LFEKAGPIWD LRLMMDPLTG LNRGYAFVTF CTKEAAQEAV KLYNNHEIRS GKHIGVCISV 240
ANNRLFVGSI PKSKTKEQIL EEFSKVTEGL TDVILYHQPD DKKKNRGFCF LEYEDHKTAA 300
QARRRLMSGK VKVWGNVGTV EWADPIEDPD PEVMAKVKVL FVRNLANTVT EEILEKSFSQ 360
FGKLERVKKL KDYAFIHFDE RDGAVKAMEE MNGKDLEGEN IEIVFAKPPD QKRKERKAQR 420
QAAKNQMYDD YYYYGPPHMP PPTRGRGRGG RGGYGYPPDY YGYEDYYDYY GYDYHNYRGG 480
YEDPYYGYED FQVGARGRGG RGARGAAPSR GRGAAPPRGR AGYSQRGGPG SARGVRGARG 540
GAQQQRGRGV RGARGGRGGN VGGKRKADGY NQPDTKRRQT NNQNWGSQPI AQQPLQGGDH 600
SGNYGYKSEN QEFYQDTFGQ QWK 623 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0070937; C:CRD-mediated mRNA stability complex; ISS:UniProtKB.
 GO:0071204; C:histone pre-mRNA 3'end processing complex; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0008143; F:poly(A) RNA binding; IDA:MGI.
 GO:0071346; P:cellular response to interferon-gamma; IEA:Compara.
 GO:0070934; P:CRD-mediated mRNA stabilization; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0017148; P:negative regulation of translation; IEA:Compara.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR006535; HnRNP_R/Q_splicing_fac.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS