UniProt Accession

 ECE2_HUMAN; O60344; A5PLK8; Q6NTG7; Q6UW36; Q8NFD7; Q96NX3; Q96NX4; Q9BRZ8 

Genbank Protein ID

 AF428263; AF428264; AF192531; AF521189; AB011176; AY359003; AC061705; AC078797; CH471052; BC005835; BC012449; BC069005; BC142950 

Genbank Nucleotide ID

 AAL30386.1; AAL30387.1; AAG28399.1; AAM77664.1; BAA25530.2; AAQ89362.1; EAW78277.1; AAH05835.1; AAH12449.1; AAH69005.1; AAI42951.1 

Protein Name

 Endothelin-converting enzyme 2 

Protein Synonyms/Alias

 ECE-2; Methyltransferase-like region; Endothelin-converting enzyme 2 region 

Gene Name

 ECE2 

Gene Synonyms/Alias

 KIAA0604; UNQ403/PRO740 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
116	WETMDVRKLDFPSAS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta- amyloid peptide accumulation in brain. May also have methyltransferase activity. 

Sequence Annotation

 REGION 1 160 Methyltransferase-like region.
 REGION 88 89 S-adenosyl-L-homocysteine binding.
 REGION 113 114 S-adenosyl-L-homocysteine binding.
 REGION 200 883 Endothelin-converting enzyme 2 region.
 ACT_SITE 721 721 By similarity.
 ACT_SITE 784 784 Proton donor (By similarity).
 METAL 720 720 Zinc; catalytic (By similarity).
 METAL 724 724 Zinc; catalytic (By similarity).
 METAL 780 780 Zinc; catalytic (By similarity).
 BINDING 30 30 S-adenosyl-L-homocysteine.
 BINDING 41 41 S-adenosyl-L-homocysteine; via amide
 BINDING 66 66 S-adenosyl-L-homocysteine; via carbonyl
 BINDING 130 130 S-adenosyl-L-homocysteine.
 MOD_RES 39 39 Phosphotyrosine.
 CARBOHYD 324 324 N-linked (GlcNAc...) (Potential).
 CARBOHYD 384 384 N-linked (GlcNAc...) (Potential).
 CARBOHYD 429 429 N-linked (GlcNAc...) (Potential).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Metal-binding; Metalloprotease; Methyltransferase; Multifunctional enzyme; Phosphoprotein; Polymorphism; Protease; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 883 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IDA:UniProtKB.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0007420; P:brain development; ISS:UniProtKB.
 GO:0010002; P:cardioblast differentiation; ISS:UniProtKB.
 GO:0007267; P:cell-cell signaling; TAS:UniProtKB.
 GO:0016486; P:peptide hormone processing; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR024079; MetalloPept_cat_dom.
 IPR013216; Methyltransf_11.
 IPR000718; Peptidase_M13.
 IPR018497; Peptidase_M13_C.
 IPR008753; Peptidase_M13_N. 

Pfam

 PF08241; Methyltransf_11
 PF01431; Peptidase_M13
 PF05649; Peptidase_M13_N 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS

 PR00786; NEPRILYSIN.