CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001441
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tripeptidyl-peptidase 1 
Protein Synonyms/Alias
 TPP-1; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I 
Gene Name
 Tpp1 
Gene Synonyms/Alias
 Cln2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
345RVNTEFMKAAARGLTacetylation[1]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 
Functional Description
 Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus (By similarity). 
Sequence Annotation
 ACT_SITE 271 271 Charge relay system (By similarity).
 ACT_SITE 275 275 Charge relay system (By similarity).
 ACT_SITE 474 474 Charge relay system (By similarity).
 METAL 516 516 Calcium (By similarity).
 METAL 517 517 Calcium; via carbonyl oxygen (By
 METAL 538 538 Calcium; via carbonyl oxygen (By
 METAL 540 540 Calcium; via carbonyl oxygen (By
 METAL 542 542 Calcium (By similarity).
 CARBOHYD 209 209 N-linked (GlcNAc...) (Potential).
 CARBOHYD 221 221 N-linked (GlcNAc...) (high mannose).
 CARBOHYD 285 285 N-linked (GlcNAc...) (Potential).
 CARBOHYD 312 312 N-linked (GlcNAc...) (Potential).
 CARBOHYD 442 442 N-linked (GlcNAc...) (Potential).
 DISULFID 111 122 By similarity.
 DISULFID 364 525 By similarity.
 DISULFID 521 536 By similarity.  
Keyword
 Autocatalytic cleavage; Calcium; Complete proteome; Disulfide bond; Glycoprotein; Hydrolase; Lysosome; Metal-binding; Protease; Reference proteome; Serine protease; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 562 AA 
Protein Sequence
MGLQARLLGL LALVIAGKCT YNPEPDQRWM LPPGWVSLGR VDPEEELSLT FALKQRNLER 60
LSELVQAVSD PSSPQYGKYL TLEDVAELVQ PSPLTLLTVQ KWLSAAGARN CDSVTTQDFL 120
TCWLSVRQAE LLLPGAEFHR YVGGPTKTHV IRSPHPYQLP QALAPHVDFV GGLHRFPPSS 180
PRQRPEPQQV GTVSLHLGVT PSVLRQRYNL TAKDVGSGTT NNSQACAQFL EQYFHNSDLT 240
EFMRLFGGSF THQASVAKVV GKQGRGRAGI EASLDVEYLM SAGANISTWV YSSPGRHEAQ 300
EPFLQWLLLL SNESSLPHVH TVSYGDDEDS LSSIYIQRVN TEFMKAAARG LTLLFASGDT 360
GAGCWSVSGR HKFRPSFPAS SPYVTTVGGT SFKNPFLITD EVVDYISGGG FSNVFPRPPY 420
QEEAVAQFLK SSSHLPPSSY FNASGRAYPD VAALSDGYWV VSNMVPIPWV SGTSASTPVF 480
GGILSLINEH RILNGRPPLG FLNPRLYQQH GTGLFDVTHG CHESCLNEEV EGQGFCSGPG 540
WDPVTGWGTP NFPALLKTLL NP 562 
Gene Ontology
 GO:0005764; C:lysosome; ISS:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004175; F:endopeptidase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
 GO:0008240; F:tripeptidyl-peptidase activity; ISS:UniProtKB.
 GO:0045453; P:bone resorption; ISS:UniProtKB.
 GO:0007040; P:lysosome organization; IMP:MGI.
 GO:0007399; P:nervous system development; ISS:UniProtKB.
 GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
 GO:0043171; P:peptide catabolic process; ISS:UniProtKB.
 GO:0006508; P:proteolysis; ISS:UniProtKB. 
Interpro
 IPR015366; Peptidase_S53_propep.
 IPR000209; Peptidase_S8/S53_dom.
 IPR009020; Prot_inh_propept. 
Pfam
 PF00082; Peptidase_S8
 PF09286; Pro-kuma_activ 
SMART
 SM00944; Pro-kuma_activ 
PROSITE
  
PRINTS