UniProt Accession

 MAP1_ECOLI; P0AE18; P07906 

Genbank Protein ID

 M15106; U70214; U00096; AP009048 

Genbank Nucleotide ID

 AAA24112.1; AAB08597.1; AAC73279.1; BAB96743.1 

Protein Name

 Methionine aminopeptidase 

Protein Synonyms/Alias

 MAP; MetAP; Peptidase M 

Gene Name

 map 

Gene Synonyms/Alias

 b0168; JW0163 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
13	KTPEDIEKMRVAGRL	acetylation	[1]
89	PDDAKLLKDGDIVNI	acetylation	[1]
117	SKMFIVGKPTIMGER	acetylation	[1]
141	YLALRMVKPGINLRE	acetylation	[1]
155	EIGAAIQKFVEAEGF	acetylation	[1]
196	RETNVVLKPGMTFTI	acetylation	[1]
218	KKEIRTMKDGWTVKT	acetylation	[1]
224	MKDGWTVKTKDRSLS	acetylation	[1]
226	DGWTVKTKDRSLSAQ	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. 

Sequence Annotation

 METAL 97 97 Divalent metal cation 1.
 METAL 108 108 Divalent metal cation 1.
 METAL 108 108 Divalent metal cation 2; catalytic.
 METAL 171 171 Divalent metal cation 2; catalytic; via
 METAL 204 204 Divalent metal cation 2; catalytic.
 METAL 235 235 Divalent metal cation 1.
 METAL 235 235 Divalent metal cation 2; catalytic.
 BINDING 79 79 Substrate.
 BINDING 99 99 Substrate.
 BINDING 178 178 Substrate.  

Keyword

 3D-structure; Aminopeptidase; Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Protease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 264 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:EcoCyc.
 GO:0008198; F:ferrous iron binding; IDA:EcoCyc.
 GO:0070006; F:metalloaminopeptidase activity; IDA:EcoCyc.
 GO:0070084; P:protein initiator methionine removal; IDA:EcoCyc.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR001714; Pept_M24_MAP.
 IPR000994; Pept_M24_structural-domain.
 IPR002467; Pept_M24A_MAP1. 

Pfam

 PF00557; Peptidase_M24 

SMART

  

PROSITE

 PS00680; MAP_1 

PRINTS

 PR00599; MAPEPTIDASE.