CPLM-018795

Genbank Nucleotide ID

Acidic leucine-rich nuclear phosphoprotein 32 family member B

Protein Synonyms/Alias

Acidic protein rich in leucines; Putative HLA-DR-associated protein I-2; PHAPI2; Silver-stainable protein SSP29

Homo sapiens (Human)

Position	Peptide	Type	References
86	GLDMLAEKLPNLTHL	acetylation	[1]
86	GLDMLAEKLPNLTHL	ubiquitination	[2]
99	HLNLSGNKLKDISTL	ubiquitination	[3, 4]
101	NLSGNKLKDISTLEP	ubiquitination	[2, 3]
110	ISTLEPLKKLECLKS	ubiquitination	[2]
111	STLEPLKKLECLKSL	ubiquitination	[2]
116	LKKLECLKSLDLFNC	ubiquitination	[2]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.

REPEAT 16 40 LRR 1.
REPEAT 43 64 LRR 2.
REPEAT 65 84 LRR 3.
REPEAT 89 110 LRR 4.
DOMAIN 123 161 LRRCT.
MOD_RES 86 86 N6-acetyllysine.
MOD_RES 244 244 Phosphothreonine.

3D-structure; Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; Leucine-rich repeat; Nucleus; Phosphoprotein; Reference proteome; Repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.

IPR001611; Leu-rich_rpt.
IPR003603; U2A'_phosphoprotein32A_C.