CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005897
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myo-inositol transporter 1 
Protein Synonyms/Alias
  
Gene Name
 ITR1 
Gene Synonyms/Alias
 YDR497C; D9719.3 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
38SEHDSPSKRGKITLEubiquitination[1]
41DSPSKRGKITLESHEubiquitination[1]
128KVLTYGEKEIVTAATubiquitination[2]
276TPRYYVMKGDLARATubiquitination[1]
301SEEIIERKVEELVTLubiquitination[1]
573VARVHELKYEPTQEIacetylation[3]
573VARVHELKYEPTQEIubiquitination[1, 2, 4, 5, 6, 7]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [5] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [6] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [7] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047
Functional Description
 Major transporter for myo-inositol. 
Sequence Annotation
 MOD_RES 12 12 Phosphothreonine.
 MOD_RES 26 26 Phosphoserine.
 MOD_RES 31 31 Phosphoserine.
 MOD_RES 35 35 Phosphoserine.
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 46 46 Phosphoserine.
 CARBOHYD 371 371 N-linked (GlcNAc...) (Potential).
 CROSSLNK 573 573 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Glycoprotein; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 584 AA 
Protein Sequence
MGIHIPYLTS KTSQSNVGDA VGNADSVEFN SEHDSPSKRG KITLESHEIQ RAPASDDEDR 60
IQIKPVNDED DTSVMITFNQ SLSPFIITLT FVASISGFMF GYDTGYISSA LISIGTDLDH 120
KVLTYGEKEI VTAATSLGAL ITSIFAGTAA DIFGRKRCLM GSNLMFVIGA ILQVSAHTFW 180
QMAVGRLIMG FGVGIGSLIA PLFISEIAPK MIRGRLTVIN SLWLTGGQLV AYGCGAGLNY 240
VNNGWRILVG LSLIPTAVQF TCLCFLPDTP RYYVMKGDLA RATEVLKRSY TDTSEEIIER 300
KVEELVTLNQ SIPGKNVPEK VWNTIKELHT VPSNLRALII GCGLQAIQQF TGWNSLMYFS 360
GTIFETVGFK NSSAVSIIVS GTNFIFTLVA FFSIDKIGRR TILLIGLPGM TMALVVCSIA 420
FHFLGIKFDG AVAVVVSSGF SSWGIVIIVF IIVFAAFYAL GIGTVPWQQS ELFPQNVRGI 480
GTSYATATNW AGSLVIASTF LTMLQNITPA GTFAFFAGLS CLSTIFCYFC YPELSGLELE 540
EVQTILKDGF NIKASKALAK KRKQQVARVH ELKYEPTQEI IEDI 584 
Gene Ontology
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005365; F:myo-inositol transmembrane transporter activity; IMP:SGD. 
Interpro
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt.
 IPR005828; Sub_transporter.
 IPR003663; Sugar/inositol_transpt.
 IPR005829; Sugar_transporter_CS. 
Pfam
 PF00083; Sugar_tr 
SMART
  
PROSITE
 PS50850; MFS
 PS00216; SUGAR_TRANSPORT_1
 PS00217; SUGAR_TRANSPORT_2 
PRINTS
 PR00171; SUGRTRNSPORT.