CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015533
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA topoisomerase 2-binding protein 1 
Protein Synonyms/Alias
 DNA topoisomerase II-beta-binding protein 1; TopBP1; DNA topoisomerase II-binding protein 1 
Gene Name
 Topbp1 
Gene Synonyms/Alias
 Kiaa0259 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
164LVAANLKKPILLPSWacetylation[1]
164LVAANLKKPILLPSWsuccinylation[1]
1284IDYCRLIKDLGGSVIacetylation[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double- stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (By similarity). May play a role in spermatogenesis. 
Sequence Annotation
 DOMAIN 101 189 BRCT 1.
 DOMAIN 195 284 BRCT 2.
 DOMAIN 353 443 BRCT 3.
 DOMAIN 551 636 BRCT 4.
 DOMAIN 644 741 BRCT 5.
 DOMAIN 902 993 BRCT 6.
 DOMAIN 1255 1347 BRCT 7.
 MOTIF 1510 1513 Nuclear localization signal (Potential).
 MOD_RES 782 782 Phosphothreonine (By similarity).
 MOD_RES 851 851 Phosphothreonine (By similarity).
 MOD_RES 888 888 Phosphoserine (By similarity).
 MOD_RES 890 890 Phosphoserine (By similarity).
 MOD_RES 1064 1064 Phosphothreonine.  
Keyword
 Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; DNA repair; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1515 AA 
Protein Sequence
MSRNDQEPFL VKFLKSSDNS ECFFKALESI KELQSEDYLQ IITDEEALKI RENDKSLYIC 60
DRFSGTVFDH LKQLGCRIVG PQVVTFCMRH QQCVPRAEHP VYNMIMSDVT VSCTSLDKDK 120
REEVHKYVQM MGGRVYRDLN VSVTHLIAGE VGSKKYLVAA NLKKPILLPS WIKTLWEKSQ 180
EKKITKYTDV NMEDFKCPIF LGCIICVTGL NGIHRKTVQQ LTAKHGGQYM GQLKMNECTH 240
LIVQEPKGQK YECARRWNVH CVTLQWFHDS IEKGFCQDES IYKAETRVEA KMVPDTSTPT 300
AQSNAESHTL ADVSHISNIN GSCVNETMFG STTSKLECSL ENLENLDISM FQAPEDLLDG 360
CRIYLCGFSG RKLDKLRRLI NSGGGVRFNQ LNEDVTHVIV GDYDDDVRQF WSKSSHRPHV 420
VGAKWLLECF TKGYILPEES YIHTNYQPAG IAVSDQPGNQ TAVLDKSGSF SKSALVPAER 480
LQQADEDLLA QYGNDDSTMV EAKLSEALEP EVGPCPGSAH REPCDDSTHI SVQEENKSSV 540
SHCILDDSTV REEGLFSQKS FLVLGFSVEN KCNIVDIIRE HAGKIVSLPS RIVADYAVVP 600
LLGCEVDVTV GEVVTNTWLV TCIDNQTLVD PKSNPLFTPV SVMSGVTPLE DCVISFSQCV 660
GAERDSLVFL ANHLGASVQE FFVRKANAKK GMLASTHLIV KEPTGSKYEA AKKWSLPAVN 720
ISWLLETARI GKRADENHFL VDNAPKQEQV LETKIPNGVS SNPDLPAHPD AHLEIHRKKA 780
VTPLDMNRFQ SRAFRAVISQ QRGQDPTFPP VRQPLTKEPS LHLDTPSKFL SKDKLFKPSF 840
DVTDALAALE TPNAASQKRK LSSPLSEVIV RNLTVALANS SRNTDSHSAS PQLKGAHLEE 900
EETRKPLDSV VVCVSKKLSK KQSELNGVAA SLGAEYRWSF DETVTHFIYQ GRANDSNREY 960
KSAKERGVHI VSEHWLLECA QEYKHLPESL YPHTYNPKMS LDINTVQDGR LCNSRAPLAV 1020
SASKDDGPDH LSVEGNETNT MGTNDKESPL LNGSGRDDCK GALTQALEMR ENFQKQLQEI 1080
MSATCIVKTP AQKTCMSRSS CNSASSTPDS ARSVRSGRSR VLEALRQSRQ AVPDVNTEPS 1140
QNEQIIWDDP TAREERARLA SNLQWPSDPT QHSELQVEIK MPDDSPSRKP VYHSEIAEQA 1200
SCVTQAPGHP GSEEPEPPVA ERPLIPEPQA PAVASPLAKP PVAPQPADKI ETQEETHRKV 1260
KKQYVFQMSS LNSQERIDYC RLIKDLGGSV IEKQCSDPSC THMVVGYPLR NEKYLASMAA 1320
GKWVLHRSYL DACKTAGRFV QEEDYEWGSS SILDALPDVT EHQQKLALAA MRWRKRIQQS 1380
QESGIVEGAF SGWKAILRVD RPREAGFKRL LQAGGAKVLS GHPEPLLKDA THLFCDFNKL 1440
KPDDCRVFIA EATAQNMVCL KTEYIADYLM LESPPCADNY RVSEAALFHN KKGGPGLPQK 1500
RKTPAENVVK RPRVH 1515 
Gene Ontology
 GO:0000794; C:condensed nuclear chromosome; IDA:MGI.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0001673; C:male germ cell nucleus; IDA:MGI.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0016605; C:PML body; IEA:Compara.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0007131; P:reciprocal meiotic recombination; ISA:MGI.
 GO:0010212; P:response to ionizing radiation; IEA:Compara. 
Interpro
 IPR001357; BRCT_dom.
 IPR026993; Topbp1. 
Pfam
 PF00533; BRCT
 PF12738; PTCB-BRCT 
SMART
 SM00292; BRCT 
PROSITE
 PS50172; BRCT 
PRINTS