CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004301
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aldose reductase 
Protein Synonyms/Alias
 AR; Aldehyde reductase; Aldo-keto reductase family 1 member B1 
Gene Name
 AKR1B1 
Gene Synonyms/Alias
 ALDR1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12LLLNNGAKMPILGLGubiquitination[1, 2, 3, 4, 5, 6]
22ILGLGTWKSPPGQVTubiquitination[5]
62VGVAIQEKLREQVVKubiquitination[5]
86LWCTYHEKGLVKGACacetylation[7]
86LWCTYHEKGLVKGACubiquitination[3, 6]
95LVKGACQKTLSDLKLacetylation[7]
195HPYLTQEKLIQYCQSubiquitination[5]
222SPDRPWAKPEDPSLLacetylation[7]
222SPDRPWAKPEDPSLLubiquitination[5, 8]
243AIAAKHNKTTAQVLIubiquitination[4, 5, 9, 10]
263RNLVVIPKSVTPERIacetylation[7, 11]
263RNLVVIPKSVTPERIubiquitination[3, 4, 5, 6, 8, 9, 10, 12, 13, 14]
308LLSCTSHKDYPFHEEacetylation[14]
308LLSCTSHKDYPFHEEubiquitination[5, 9, 13]
Reference
 [1] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [11] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [12] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [13] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [14] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. 
Sequence Annotation
 NP_BIND 10 19 NADP (Potential).
 NP_BIND 211 273 NADP.
 ACT_SITE 49 49 Proton donor.
 BINDING 111 111 Substrate.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 95 95 N6-acetyllysine.
 MOD_RES 222 222 N6-acetyllysine.
 MOD_RES 263 263 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 316 AA 
Protein Sequence
MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ 60
EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK 120
EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP 180
AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK 240
HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA 300
LLSCTSHKDY PFHEEF 316 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005615; C:extracellular space; TAS:ProtInc.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; TAS:ProtInc.
 GO:0009055; F:electron carrier activity; TAS:UniProtKB.
 GO:0043795; F:glyceraldehyde oxidoreductase activity; IDA:UniProtKB.
 GO:0006700; P:C21-steroid hormone biosynthetic process; TAS:Reactome.
 GO:0005975; P:carbohydrate metabolic process; TAS:ProtInc.
 GO:0044597; P:daunorubicin metabolic process; IMP:UniProtKB.
 GO:0044598; P:doxorubicin metabolic process; IMP:UniProtKB.
 GO:0006950; P:response to stress; TAS:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 
Pfam
 PF00248; Aldo_ket_red 
SMART
  
PROSITE
 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 
PRINTS
 PR00069; ALDKETRDTASE.