CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030508
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase 3 
Protein Synonyms/Alias
 Mitogen-activated protein kinase 3, isoform CRA_b; cDNA FLJ33690 fis, clone BRAWH2002967, highly similar to Mitogen-activated protein kinase 3 (EC 2.7.11.24) 
Gene Name
 MAPK3 
Gene Synonyms/Alias
 hCG_1983753 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67INTTCDLKICDFGLAubiquitination[1, 2]
173YLQSLPSKTKVAWAKubiquitination[3]
175QSLPSKTKVAWAKLFubiquitination[3]
180KTKVAWAKLFPKSDSubiquitination[3, 4, 5]
188LFPKSDSKALDLLDRubiquitination[3, 5]
203MLTFNPNKRITVEEAubiquitination[3, 5]
247DLPKERLKELIFQETubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 265 AA 
Protein Sequence
MRDVYIVQDL METDLYKLLK SQQLSNDHIC YFLYQILRGL KYIHSANVLH RDLKPSNLLI 60
NTTCDLKICD FGLARIADPE HDHTGFLTEY VATRWYRAPE IMLNSKGYTK SIDIWSVGCI 120
LAEMLSNRPI FPGKHYLDQL NHILGILGSP SQEDLNCIIN MKARNYLQSL PSKTKVAWAK 180
LFPKSDSKAL DLLDRMLTFN PNKRITVEEA LAHPYLEQYY DPTDEPVAEE PFTFAMELDD 240
LPKERLKELI FQETARFQPG VLEAP 265 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0031143; C:pseudopodium; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004707; F:MAP kinase activity; IEA:InterPro.
 GO:0051216; P:cartilage development; IEA:Compara.
 GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Compara.
 GO:0000165; P:MAPK cascade; IEA:GOC.
 GO:2000657; P:negative regulation of apolipoprotein binding; IEA:Compara.
 GO:0009887; P:organ morphogenesis; IEA:Compara.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0006974; P:response to DNA damage stimulus; IEA:Compara.
 GO:0043330; P:response to exogenous dsRNA; IEA:Compara.
 GO:0019233; P:sensory perception of pain; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR008349; MAPK_ERK1/2.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS
 PR01770; ERK1ERK2MAPK.