UniProt Accession

 F8VR31_HUMAN; F8VR31 

Genbank Protein ID

 AL136233 

Genbank Nucleotide ID

  

Protein Name

 DNA replication ATP-dependent helicase/nuclease DNA2 

Protein Synonyms/Alias

  

Gene Name

 DNA2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
28	LPAELFQKKVVASFP	ubiquitination	[1, 2, 3, 4]
29	PAELFQKKVVASFPR	ubiquitination	[3, 5]
58	AVNTVQNKEGNCEKR	ubiquitination	[3, 5]
77	ASQSLENKELCILRN	ubiquitination	[3, 5]
178	NNSFAPEKLQELAFQ	ubiquitination	[4]
194	IQEIRHLKEMYRLNL	ubiquitination	[3]
227	WAGDFMHKNTSTDFP	ubiquitination	[3, 5]
257	TCNIEVVKPMDIEES	ubiquitination	[3]
275	PRFGLKGKIDVTVGV	ubiquitination	[5]
293	RGYKTKYKIMPLELK	ubiquitination	[3, 5]
300	KIMPLELKTGKESNS	ubiquitination	[1, 3]
303	PLELKTGKESNSIEH	ubiquitination	[1]
351	VPANHLDKRELLKLR	ubiquitination	[1, 3, 5]
377	SKSATRQKTQLASLP	ubiquitination	[1, 3, 5]
391	PQIIEEEKTCKYCSQ	ubiquitination	[3, 5]
394	IEEEKTCKYCSQIGN	ubiquitination	[2, 3, 5]
425	VPIVMLPKIEEETQH	ubiquitination	[3]
459	SQSKDNKKNHQNIWL	ubiquitination	[3]
474	MPASEMEKSGSCIGN	ubiquitination	[3]
489	LIRMEHVKIVCDGQY	ubiquitination	[2, 3, 5]
503	YLHNFQCKHGAIPVT	ubiquitination	[3, 4]
536	ALSRGYVKEINMTTV	ubiquitination	[3]
567	FRLDQEEKNCDIDTP	ubiquitination	[3, 5]
580	TPLGNLSKLMENTFV	ubiquitination	[3]
589	MENTFVSKKLRDLII	ubiquitination	[3]
590	ENTFVSKKLRDLIID	ubiquitination	[3]
624	DTVACILKGLNKPQR	ubiquitination	[3, 5, 6]
641	MKKVLLSKDYTLIVG	ubiquitination	[3]
710	APYRQQLKIINDLLA	ubiquitination	[3]
730	VEVNTVDKYQGRDKS	ubiquitination	[1, 5, 6]
757	GTVGELLKDWRRLNV	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 801 AA 

Protein Sequence

Gene Ontology

 GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
 GO:0043142; F:single-stranded DNA-dependent ATPase activity; IEA:InterPro.
 GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:InterPro. 

Interpro

 IPR026851; Dna2.
 IPR014808; DNA_replication_fac_Dna2_N.
 IPR027417; P-loop_NTPase. 

Pfam

 PF08696; Dna2 

SMART

  

PROSITE

  

PRINTS