CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015609
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 34 
Protein Synonyms/Alias
 Deubiquitinating enzyme 34; Ubiquitin thioesterase 34; Ubiquitin-specific-processing protease 34 
Gene Name
 USP34 
Gene Synonyms/Alias
 KIAA0570; KIAA0729 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
333AAKAQLSKQSSFASLubiquitination[1]
1423KAAGDHAKGLHIPRLubiquitination[1, 2]
1817CKTYTMDKQPLNTGEubiquitination[1, 2]
1917TCSHCGKKVRAEKRAubiquitination[2]
1949FNMVTMMKEKVNTHFubiquitination[1, 2]
1951MVTMMKEKVNTHFSFubiquitination[1]
2068TYDSVTDKFMDFSFEubiquitination[1]
2357ILSLAEEKYRPAALEubiquitination[2]
3202DEGATPIKRRRVSSDubiquitination[2]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. 
Sequence Annotation
 ACT_SITE 1903 1903 Nucleophile (Probable).
 ACT_SITE 2164 2164 Proton acceptor (By similarity).
 MOD_RES 352 352 Phosphoserine.
 MOD_RES 649 649 Phosphoserine.
 MOD_RES 2488 2488 Phosphoserine.
 MOD_RES 2545 2545 Phosphothreonine (By similarity).
 MOD_RES 3358 3358 Phosphoserine.
 MOD_RES 3359 3359 Phosphoserine.
 MOD_RES 3406 3406 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Hydrolase; Phosphoprotein; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 3546 AA 
Protein Sequence
MRRKNSYYVW QKIFQIQFPL YTAYKHNTHP TIEDISTQES NILGAFCDMN DVEVPLHLLR 60
YVCLFCGKNG LSLMKDCFEY GTPETLPFLI AHAFITVVSN IRIWLHIPAV MQHIIPFRTY 120
VIRYLCKLSD QELRQSAARN MADLMWSTVK EPLDTTLCFD KESLDLAFKY FMSPTLTMRL 180
AGLSQITNQL HTFNDVCNNE SLVSDTETSI AKELADWLIS NNVVEHIFGP NLHIEIIKQC 240
QVILNFLAAE GRLSTQHIDC IWAAAQLKHC SRYIHDLFPS LIKNLDPVPL RHLLNLVSAL 300
EPSVHTEQTL YLASMLIKAL WNNALAAKAQ LSKQSSFASL LNTNIPIGNK KEEEELRRTA 360
PSPWSPAASP QSSDNSDTHQ SGGSDIEMDE QLINRTKHVQ QRLSDTEESM QGSSDETANS 420
GEDGSSGPGS SSGHSDGSSN EVNSSHASQS AGSPGSEVQS EDIADIEALK EEDEDDDHGH 480
NPPKSSCGTD LRNRKLESQA GICLGDSQGM SERNGTSSGT GKDLVFNTES LPSVDNRMRM 540
LDACSHSEDP EHDISGEMNA THIAQGSQES CITRTGDFLG ETIGNELFNC RQFIGPQHHH 600
HHHHHHHHHD GHMVDDMLSA DDVSCSSSQV SAKSEKNMAD FDGEESGCEE ELVQINSHAE 660
LTSHLQQHLP NLASIYHEHL SQGPVVHKHQ FNSNAVTDIN LDNVCKKGNT LLWDIVQDED 720
AVNLSEGLIN EAEKLLCSLV CWFTDRQIRM RFIEGCLENL GNNRSVVISL RLLPKLFGTF 780
QQFGSSYDTH WITMWAEKEL NMMKLFFDNL VYYIQTVREG RQKHALYSHS AEVQVRLQFL 840
TCVFSTLGSP DHFRLSLEQV DILWHCLVED SECYDDALHW FLNQVRSKDQ HAMGMETYKH 900
LFLEKMPQLK PETISMTGLN LFQHLCNLAR LATSAYDGCS NSELCGMDQF WGIALRAQSG 960
DVSRAAIQYI NSYYINGKTG LEKEQEFISK CMESLMIASS SLEQESHSSL MVIERGLLML 1020
KTHLEAFRRR FAYHLRQWQI EGTGISSHLK ALSDKQSLPL RVVCQPAGLP DKMTIEMYPS 1080
DQVADLRAEV THWYENLQKE QINQQAQLQE FGQSNRKGEF PGGLMGPVRM ISSGHELTTD 1140
YDEKALHELG FKDMQMVFVS LGAPRRERKG EGVQLPASCL PPPQKDNIPM LLLLQEPHLT 1200
TLFDLLEMLA SFKPPSGKVA VDDSESLRCE ELHLHAENLS RRVWELLMLL PTCPNMLMAF 1260
QNISDEQSND GFNWKELLKI KSAHKLLYAL EIIEALGKPN RRIRRESTGS YSDLYPDSDD 1320
SSEDQVENSK NSWSCKFVAA GGLQQLLEIF NSGILEPKEQ ESWTVWQLDC LACLLKLICQ 1380
FAVDPSDLDL AYHDVFAWSG IAESHRKRTW PGKSRKAAGD HAKGLHIPRL TEVFLVLVQG 1440
TSLIQRLMSV AYTYDNLAPR VLKAQSDHRS RHEVSHYSMW LLVSWAHCCS LVKSSLADSD 1500
HLQDWLKKLT LLIPETAVRH ESCSGLYKLS LSGLDGGDSI NRSFLLLAAS TLLKFLPDAQ 1560
ALKPIRIDDY EEEPILKPGC KEYFWLLCKL VDNIHIKDAS QTTLLDLDAL ARHLADCIRS 1620
REILDHQDGN VEDDGLTGLL RLATSVVKHK PPFKFSREGQ EFLRDIFNLL FLLPSLKDRQ 1680
QPKCKSHSSR AAAYDLLVEM VKGSVENYRL IHNWVMAQHM QSHAPYKWDY WPHEDVRAEC 1740
RFVGLTNLGA TCYLASTIQQ LYMIPEARQA VFTAKYSEDM KHKTTLLELQ KMFTYLMESE 1800
CKAYNPRPFC KTYTMDKQPL NTGEQKDMTE FFTDLITKIE EMSPELKNTV KSLFGGVITN 1860
NVVSLDCEHV SQTAEEFYTV RCQVADMKNI YESLDEVTIK DTLEGDNMYT CSHCGKKVRA 1920
EKRACFKKLP RILSFNTMRY TFNMVTMMKE KVNTHFSFPL RLDMTPYTED FLMGKSERKE 1980
GFKEVSDHSK DSESYEYDLI GVTVHTGTAD GGHYYSFIRD IVNPHAYKNN KWYLFNDAEV 2040
KPFDSAQLAS ECFGGEMTTK TYDSVTDKFM DFSFEKTHSA YMLFYKRMEP EEENGREYKF 2100
DVSSELLEWI WHDNMQFLQD KNIFEHTYFG FMWQLCSCIP STLPDPKAVS LMTAKLSTSF 2160
VLETFIHSKE KPTMLQWIEL LTKQFNNSQA ACEWFLDRMA DDDWWPMQIL IKCPNQIVRQ 2220
MFQRLCIHVI QRLRPVHAHL YLQPGMEDGS DDMDTSVEDI GGRSCVTRFV RTLLLIMEHG 2280
VKPHSKHLTE YFAFLYEFAK MGEEESQFLL SLQAISTMVH FYMGTKGPEN PQVEVLSEEE 2340
GEEEEEEEDI LSLAEEKYRP AALEKMIALV ALLVEQSRSE RHLTLSQTDM AALTGGKGFP 2400
FLFQHIRDGI NIRQTCNLIF SLCRYNNRLA EHIVSMLFTS IAKLTPEAAN PFFKLLTMLM 2460
EFAGGPPGMP PFASYILQRI WEVIEYNPSQ CLDWLAVQTP RNKLAHSWVL QNMENWVERF 2520
LLAHNYPRVR TSAAYLLVSL IPSNSFRQMF RSTRSLHIPT RDLPLSPDTT VVLHQVYNVL 2580
LGLLSRAKLY VDAAVHGTTK LVPYFSFMTY CLISKTEKLM FSTYFMDLWN LFQPKLSEPA 2640
IATNHNKQAL LSFWYNVCAD CPENIRLIVQ NPVVTKNIAF NYILADHDDQ DVVLFNRGML 2700
PAYYGILRLC CEQSPAFTRQ LASHQNIQWA FKNLTPHASQ YPGAVEELFN LMQLFIAQRP 2760
DMREEELEDI KQFKKTTISC YLRCLDGRSC WTTLISAFRI LLESDEDRLL VVFNRGLILM 2820
TESFNTLHMM YHEATACHVT GDLVELLSIF LSVLKSTRPY LQRKDVKQAL IQWQERIEFA 2880
HKLLTLLNSY SPPELRNACI DVLKELVLLS PHDFLHTLVP FLQHNHCTYH HSNIPMSLGP 2940
YFPCRENIKL IGGKSNIRPP RPELNMCLLP TMVETSKGKD DVYDRMLLDY FFSYHQFIHL 3000
LCRVAINCEK FTETLVKLSV LVAYEGLPLH LALFPKLWTE LCQTQSAMSK NCIKLLCEDP 3060
VFAEYIKCIL MDERTFLNNN IVYTFMTHFL LKVQSQVFSE ANCANLISTL ITNLISQYQN 3120
LQSDFSNRVE ISKASASLNG DLRALALLLS VHTPKQLNPA LIPTLQELLS KCRTCLQQRN 3180
SLQEQEAKER KTKDDEGATP IKRRRVSSDE EHTVDSCISD MKTETREVLT PTSTSDNETR 3240
DSSIIDPGTE QDLPSPENSS VKEYRMEVPS SFSEDMSNIR SQHAEEQSNN GRYDDCKEFK 3300
DLHCSKDSTL AEEESEFPST SISAVLSDLA DLRSCDGQAL PSQDPEVALS LSCGHSRGLF 3360
SHMQQHDILD TLCRTIESTI HVVTRISGKG NQAAS 3395 
Gene Ontology
 GO:0004197; F:cysteine-type endopeptidase activity; IMP:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IDA:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0071108; P:protein K48-linked deubiquitination; IDA:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR016024; ARM-type_fold.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS