UniProt Accession

 DNJC2_HUMAN; Q99543; A4VCI0; Q9BVX1 

Genbank Protein ID

 X98260; AC004668; BC000859; BC139751 

Genbank Nucleotide ID

 CAA66913.1; AAH00859.1; AAI39752.1 

Protein Name

 DnaJ homolog subfamily C member 2 

Protein Synonyms/Alias

 M-phase phosphoprotein 11; Zuotin-related factor 1 

Gene Name

 DNAJC2 

Gene Synonyms/Alias

 MPHOSPH11; MPP11; ZRF1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
40	RWFEAFVKRRNRNAS	ubiquitination	[1]
514	QKLDPHQKDDINKKA	ubiquitination	[2]
567	KLLEQALKTYPVNTP	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 

Sequence Annotation

 DOMAIN 88 161 J.
 DOMAIN 449 511 SANT 1.
 DOMAIN 549 604 SANT 2.
 REGION 23 31 Epitope (recognized by CD8(+) cytotoxic
 REGION 160 250 ZRF1-UBD.
 MOD_RES 47 47 Phosphoserine.
 MOD_RES 49 49 Phosphoserine.
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 63 63 Phosphoserine.  

Keyword

 3D-structure; Activator; Alternative splicing; Chaperone; Chromatin regulator; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 621 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0042393; F:histone binding; IDA:UniProtKB.
 GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
 GO:0051083; P:'de novo' cotranslational protein folding; TAS:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:Compara.
 GO:0000085; P:G2 phase of mitotic cell cycle; IEA:Compara.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR009057; Homeodomain-like.
 IPR001005; SANT/Myb.
 IPR017884; SANT_dom. 

Pfam

 PF00226; DnaJ
 PF00249; Myb_DNA-binding 

SMART

 SM00271; DnaJ
 SM00717; SANT 

PROSITE

 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS51293; SANT 

PRINTS