UniProt Accession

 UBP14_MOUSE; Q9JMA1; Q543U5; Q923F2; Q9D0L0 

Genbank Protein ID

 AB034633; AK011322; AK029977; AK045909; BC005571 

Genbank Nucleotide ID

 BAA93551.1; BAB27544.1; BAC26713.1; BAC32528.1; AAH05571.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase 14 

Protein Synonyms/Alias

 Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14 

Gene Name

 Usp14 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
14	TVKWGKEKFEGVELN	ubiquitination	[1]
49	ARQKVMVKGGTLKDD	ubiquitination	[1]
62	DDDWGNIKMKNGMTV	ubiquitination	[1]
64	DWGNIKMKNGMTVLM	ubiquitination	[1]
130	IRSVPELKDALKRYA	ubiquitination	[1]
214	MMRVLQQKLEAIEDD	ubiquitination	[1]
238	APAVTPSKKKSLIDQ	ubiquitination	[1]
291	KYLFTGLKLRLQEEI	ubiquitination	[1]
300	RLQEEITKQSPTLQR	ubiquitination	[1]
313	QRNALYIKSSKISRL	ubiquitination	[1]
342	EKESVNAKVLKDVKF	ubiquitination	[1]
448	RKQDEWIKFDDDKVS	ubiquitination	[1]
453	WIKFDDDKVSIVTPE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Maintains the cellular levels of monomeric ubiquitin in cells. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor cxcr4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. 

Sequence Annotation

 DOMAIN 4 80 Ubiquitin-like.
 ACT_SITE 114 114 Nucleophile (By similarity).
 ACT_SITE 434 434 Proton acceptor (By similarity).
 MOD_RES 136 136 Phosphotyrosine.
 MOD_RES 143 143 Phosphoserine.
 MOD_RES 222 222 Phosphoserine.
 MOD_RES 235 235 Phosphothreonine (By similarity).
 MOD_RES 448 448 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Cell membrane; Complete proteome; Cytoplasm; Hydrolase; Membrane; Phosphoprotein; Protease; Proteasome; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 493 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:Compara.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0045202; C:synapse; IDA:MGI.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004866; F:endopeptidase inhibitor activity; IEA:Compara.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:Compara.
 GO:0050920; P:regulation of chemotaxis; IEA:Compara.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IEA:Compara.
 GO:0007268; P:synaptic transmission; IMP:MGI.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF00443; UCH 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 

PRINTS