CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012154
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Diacylglycerol kinase zeta 
Protein Synonyms/Alias
 DAG kinase zeta; Diglyceride kinase zeta; DGK-zeta 
Gene Name
 DGKZ 
Gene Synonyms/Alias
 DAGK6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
231RQVALRRKAAGPQAWubiquitination[1, 2]
336PCIEQLEKINFRCKPubiquitination[1]
559TLDQLRLKPPPPVAIubiquitination[1]
662SREANPEKFNSRFRNubiquitination[1]
691GSSKDLAKHIRVVCDubiquitination[1]
710TPKIQDLKPQCVVFLubiquitination[1]
794QVDGEPCKLAASRIRubiquitination[1]
893EPDGAGAKSPTCQKLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. 
Sequence Annotation
 DOMAIN 480 614 DAGKc.
 REPEAT 1011 1041 ANK 1.
 REPEAT 1046 1075 ANK 2.
 ZN_FING 287 341 Phorbol-ester/DAG-type 1.
 ZN_FING 361 419 Phorbol-ester/DAG-type 2.
 REGION 448 462 MARCKS homology.
 REGION 467 605 Mediates interaction with RASGRP1.
 MOTIF 550 558 Nuclear export signal (By similarity).  
Keyword
 Alternative splicing; ANK repeat; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1117 AA 
Protein Sequence
METFFRRHFR GKVPGPGEGQ QRPSSVGLPT GKARRRSPAG QASSSLAQRR RSSAQLQGCL 60
LSCGVRAQGS SRRRSSTVPP SCNPRFIVDK VLTPQPTTVG AQLLGAPLLL TGLVGMNEEE 120
GVQEDVVAEA SSAIQPGTKT PGPPPPRGAQ PLLPLPRYLR RASSHLLPAD AVYDHALWGL 180
HGYYRRLSQR RPSGQHPGPG GRRASGTTAG TMLPTRVRPL SRRRQVALRR KAAGPQAWSA 240
LLAKAITKSG LQHLAPPPPT PGAPCSESER QIRSTVDWSE SATYGEHIWF ETNVSGDFCY 300
VGEQYCVARM LKSVSRRKCA ACKIVVHTPC IEQLEKINFR CKPSFRESGS RNVREPTFVR 360
HHWVHRRRQD GKCRHCGKGF QQKFTFHSKE IVAISCSWCK QAYHSKVSCF MLQQIEEPCS 420
LGVHAAVVIP PTWILRARRP QNTLKASKKK KRASFKRKSS KKGPEEGRWR PFIIRPTPSP 480
LMKPLLVFVN PKSGGNQGAK IIQSFLWYLN PRQVFDLSQG GPKEALEMYR KVHNLRILAC 540
GGDGTVGWIL STLDQLRLKP PPPVAILPLG TGNDLARTLN WGGGYTDEPV SKILSHVEEG 600
NVVQLDRWDL HAEPNPEAGP EDRDEGATDR LPLDVFNNYF SLGFDAHVTL EFHESREANP 660
EKFNSRFRNK MFYAGTAFSD FLMGSSKDLA KHIRVVCDGM DLTPKIQDLK PQCVVFLNIP 720
RYCAGTMPWG HPGEHHDFEP QRHDDGYLEV IGFTMTSLAA LQVGGHGERL TQCREVVLTT 780
SKAIPVQVDG EPCKLAASRI RIALRNQATM VQKAKRRSAA PLHSDQQPVP EQLRIQVSRV 840
SMHDYEALHY DKEQLKEASV PLGTVVVPGD SDLELCRAHI ERLQQEPDGA GAKSPTCQKL 900
SPKWCFLDAT TASRFYRIDR AQEHLNYVTE IAQDEIYILD PELLGASARP DLPTPTSPLP 960
TSPCSPTPRS LQGDAAPPQG EELIEAAKRN DFCKLQELHR AGGDLMHRDE QSRTLLHHAV 1020
STGSKDVVRY LLDHAPPEIL DAVEENGETC LHQAAALGQR TICHYIVEAG ASLMKTDQQG 1080
DTPRQRAEKA QDTELAAYLE NRQHYQMIQR EDQETAV 1117 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0004143; F:diacylglycerol kinase activity; IDA:UniProtKB.
 GO:0004857; F:enzyme inhibitor activity; IEA:Compara.
 GO:0001727; F:lipid kinase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008022; F:protein C-terminus binding; IDA:UniProtKB.
 GO:0016477; P:cell migration; NAS:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0031571; P:mitotic G1 DNA damage checkpoint; IGI:UniProtKB.
 GO:0045930; P:negative regulation of mitotic cell cycle; IGI:UniProtKB.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IEA:Compara.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR000756; Diacylglycerol_kin_accessory.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd. 
Pfam
 PF00023; Ank
 PF00130; C1_1
 PF00609; DAGK_acc
 PF00781; DAGK_cat 
SMART
 SM00248; ANK
 SM00109; C1
 SM00045; DAGKa
 SM00046; DAGKc 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50146; DAGK
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS