UniProt Accession

 MFGM_HUMAN; Q08431; B2R6M7; Q53FU9; Q7Z3D2; Q9BTL9 

Genbank Protein ID

 U58516; AY141974; BT006948; AK312640; AK223182; AK222735; BX537974; AC067805; CH471101; BC003610; S56151 

Genbank Nucleotide ID

 AAC50549.1; AAN08508.1; AAP35594.1; BAG35524.1; BAD96902.1; BAD96455.1; CAD97938.1; EAX02026.1; AAH03610.1; AAB19771.1 

Protein Name

 Lactadherin 

Protein Synonyms/Alias

 Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1; Lactadherin short form; Medin 

Gene Name

 MFGE8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
59	SYTCTCLKGYAGNHC	ubiquitination	[1]
69	AGNHCETKCVEPLGM	ubiquitination	[1]
156	LASHEYLKAFKVAYS	ubiquitination	[2, 3]
177	DFIHDVNKKHKEFVG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 

Sequence Annotation

 DOMAIN 24 67 EGF-like.
 DOMAIN 70 225 F5/8 type C 1.
 DOMAIN 230 387 F5/8 type C 2.
 MOTIF 46 48 Cell attachment site.
 CARBOHYD 228 228 N-linked (GlcNAc...); atypical.
 CARBOHYD 238 238 N-linked (GlcNAc...).
 CARBOHYD 325 325 N-linked (GlcNAc...).
 CARBOHYD 329 329 N-linked (GlcNAc...).
 CARBOHYD 350 350 N-linked (GlcNAc...).
 DISULFID 27 38 By similarity.
 DISULFID 32 55 By similarity.
 DISULFID 57 66 By similarity.
 DISULFID 70 225 By similarity.
 DISULFID 212 216 By similarity.
 DISULFID 230 387 By similarity.  

Keyword

 Alternative splicing; Amyloid; Angiogenesis; Cell adhesion; Complete proteome; Direct protein sequencing; Disulfide bond; EGF-like domain; Fertilization; Glycoprotein; Host-virus interaction; Membrane; Polymorphism; Reference proteome; Repeat; Secreted; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 387 AA 

Protein Sequence

Gene Ontology

 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB.
 GO:0019897; C:extrinsic to plasma membrane; IEA:Compara.
 GO:0008429; F:phosphatidylethanolamine binding; IEA:Compara.
 GO:0001786; F:phosphatidylserine binding; IEA:Compara.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0006911; P:phagocytosis, engulfment; IEA:Compara.
 GO:0006910; P:phagocytosis, recognition; IEA:Compara.
 GO:2000427; P:positive regulation of apoptotic cell clearance; IEA:Compara.
 GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR000421; Coagulation_fac_5/8-C_type_dom.
 IPR000742; EG-like_dom.
 IPR013032; EGF-like_CS.
 IPR008979; Galactose-bd-like.
 IPR027060; Lactadherin. 

Pfam

 PF00008; EGF
 PF00754; F5_F8_type_C 

SMART

 SM00181; EGF
 SM00231; FA58C 

PROSITE

 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3
 PS01285; FA58C_1
 PS01286; FA58C_2
 PS50022; FA58C_3 

PRINTS