UniProt Accession

 EYA3_HUMAN; Q99504; A8K190; O95463; Q99813 

Genbank Protein ID

 Y10262; U81602; AK289805; AL512288; AL137792; CH471059; AJ007991 

Genbank Nucleotide ID

 CAA71311.1; AAB42066.1; BAF82494.1; CAI14297.1; CAI14297.1; EAX07713.1; CAA07814.1 

Protein Name

 Eyes absent homolog 3 

Protein Synonyms/Alias

  

Gene Name

 EYA3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
272	LSQTTPSKDTDDQSR	ubiquitination	[1]
515	YSATKIGKESCFERI	ubiquitination	[1]
546	RDEEIAAKQHNMPFW	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye. 

Sequence Annotation

 ACT_SITE 309 309 Nucleophile (Probable).
 ACT_SITE 311 311 Proton donor (By similarity).
 METAL 309 309 Magnesium.
 METAL 311 311 Magnesium (By similarity).
 METAL 537 537 Magnesium (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 266 266 Phosphoserine.
 MOD_RES 472 472 Phosphoserine.  

Keyword

 Acetylation; Activator; Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; Developmental protein; DNA damage; DNA repair; Hydrolase; Magnesium; Metal-binding; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 573 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004725; F:protein tyrosine phosphatase activity; IDA:UniProtKB.
 GO:0008138; F:protein tyrosine/serine/threonine phosphatase activity; IEA:Compara.
 GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc.
 GO:0006302; P:double-strand break repair; IMP:UniProtKB.
 GO:0016576; P:histone dephosphorylation; IDA:UniProtKB.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0045739; P:positive regulation of DNA repair; IMP:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0010212; P:response to ionizing radiation; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007601; P:visual perception; TAS:ProtInc. 

Interpro

 IPR006545; EYA. 

Pfam

  

SMART

  

PROSITE

  

PRINTS