CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010882
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exosome component 10 
Protein Synonyms/Alias
 Autoantigen PM/Scl 2; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; Polymyositis/scleroderma autoantigen 100 kDa; PM/Scl-100; Polymyositis/scleroderma autoantigen 2 
Gene Name
 EXOSC10 
Gene Synonyms/Alias
 PMSCL; PMSCL2; RRP6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19LSATSATKSDGEMVLubiquitination[1]
37PDADSFVKFALGSVVacetylation[2]
136DEASGVNKNQQPVLPubiquitination[1, 3, 4]
150PAGLQVPKTVVSSWNubiquitination[4]
186NIIRPQLKFREKIDNubiquitination[4]
210FIKPNAQKPLPQALSubiquitination[4]
417YCNVDSNKQYQLADWubiquitination[3, 4]
680PLTVAQKKAQNIMESubiquitination[4, 5]
710APVSQAAKFDPSTKIubiquitination[3, 4, 5]
716AKFDPSTKIYEISNRubiquitination[5]
725YEISNRWKLAQVQVQubiquitination[4]
835FAGNSKSKVSSQFDPmethylation[6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA. 
Sequence Annotation
 DOMAIN 503 583 HRDC.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Exonuclease; Exosome; Hydrolase; Nonsense-mediated mRNA decay; Nuclease; Nucleus; Reference proteome; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 885 AA 
Protein Sequence
MAPPSTREPR VLSATSATKS DGEMVLPGFP DADSFVKFAL GSVVAVTKAS GGLPQFGDEY 60
DFYRSFPGFQ AFCETQGDRL LQCMSRVMQY HGCRSNIKDR SKVTELEDKF DLLVDANDVI 120
LERVGILLDE ASGVNKNQQP VLPAGLQVPK TVVSSWNRKA AEYGKKAKSE TFRLLHAKNI 180
IRPQLKFREK IDNSNTPFLP KIFIKPNAQK PLPQALSKER RERPQDRPED LDVPPALADF 240
IHQQRTQQVE QDMFAHPYQY ELNHFTPADA VLQKPQPQLY RPIEETPCHF ISSLDELVEL 300
NEKLLNCQEF AVDLEHHSYR SFLGLTCLMQ ISTRTEDFII DTLELRSDMY ILNESLTDPA 360
IVKVFHGADS DIEWLQKDFG LYVVNMFDTH QAARLLNLGR HSLDHLLKLY CNVDSNKQYQ 420
LADWRIRPLP EEMLSYARDD THYLLYIYDK MRLEMWERGN GQPVQLQVVW QRSRDICLKK 480
FIKPIFTDES YLELYRKQKK HLNTQQLTAF QLLFAWRDKT ARREDESYGY VLPNHMMLKI 540
AEELPKEPQG IIACCNPVPP LVRQQINEMH LLIQQAREMP LLKSEVAAGV KKSGPLPSAE 600
RLENVLFGPH DCSHAPPDGY PIIPTSGSVP VQKQASLFPD EKEDNLLGTT CLIATAVITL 660
FNEPSAEDSK KGPLTVAQKK AQNIMESFEN PFRMFLPSLG HRAPVSQAAK FDPSTKIYEI 720
SNRWKLAQVQ VQKDSKEAVK KKAAEQTAAR EQAKEACKAA AEQAISVRQQ VVLENAAKKR 780
ERATSDPRTT EQKQEKKRLK ISKKPKDPEP PEKEFTPYDY SQSDFKAFAG NSKSKVSSQF 840
DPNKQTPSGK KCIAAKKIKQ SVGNKSMSFP TGKSDRGFRY NWPQR 885 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0000178; C:exosome (RNase complex); IDA:UniProtKB.
 GO:0000176; C:nuclear exosome (RNase complex); IEA:InterPro.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0035327; C:transcriptionally active chromatin; IMP:UniProtKB.
 GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
 GO:0004532; F:exoribonuclease activity; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0071034; P:CUT catabolic process; IMP:UniProtKB.
 GO:0009048; P:dosage compensation by inactivation of X chromosome; IEA:Compara.
 GO:0071044; P:histone mRNA catabolic process; IMP:UniProtKB.
 GO:0000460; P:maturation of 5.8S rRNA; IMP:UniProtKB.
 GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:UniProtKB.
 GO:0071048; P:nuclear retention of unspliced pre-mRNA at the site of transcription; IMP:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:UniProtKB. 
Interpro
 IPR002562; 3'-5'_exonuclease_dom.
 IPR012588; Exosome-assoc_fac_Rrp6_N.
 IPR010997; HRDC-like.
 IPR002121; HRDC_dom.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF01612; DNA_pol_A_exo1
 PF00570; HRDC
 PF08066; PMC2NT 
SMART
 SM00474; 35EXOc
 SM00341; HRDC 
PROSITE
 PS50967; HRDC 
PRINTS