CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029163
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG-binding domain protein 1 
Protein Synonyms/Alias
 cDNA FLJ54972, highly similar to Homo sapiens pericentriolar material 1 (PCM1), mRNA 
Gene Name
 PCM1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
25TEEPVQAKVFSRKNHubiquitination[1, 2, 3, 4, 5, 6]
37KNHEQLEKIIKCNRSubiquitination[5]
139HISESHEKGENVKSVubiquitination[2]
230MREYERMKTEAESNSubiquitination[2, 5, 6]
301RQIKAIMKEVIPFLKubiquitination[1, 2, 5, 6]
308KEVIPFLKEHMDEVCubiquitination[5]
338TQQNDESKEFVKFFHubiquitination[2, 5, 6]
342DESKEFVKFFHKQLGubiquitination[5]
346EFVKFFHKQLGSILQubiquitination[1, 5, 6]
358ILQDSLAKFAGRKLKubiquitination[1, 2, 5, 6]
400DNNSITVKQRCKRKIubiquitination[1, 2, 3, 4, 5, 6, 7]
406VKQRCKRKIEATGVIubiquitination[5, 6]
549FKKTAESKNVPLEREubiquitination[2, 5, 6]
569DQNNCPVKPCYLNILubiquitination[6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 723 AA 
Protein Sequence
MSSTCEPCKS RNRHSAQTEE PVQAKVFSRK NHEQLEKIIK CNRSTEISSA HARRILQQSN 60
RNACNEAPET GSDFSMFEAL RDTIYSEVAT LISQNESRPH FLIELFHELQ LLNTDYLRQR 120
ALYALQDIVS RHISESHEKG ENVKSVNSGT WIASNSELTP SESLATTDDE TFEKNFERET 180
HKISEQNDAD NASVLSVSSN FEPFATDDLG NTVIHLDQAL ARMREYERMK TEAESNSNMR 240
CTCRIIEDGD GAGAGTTVNN LEETPVIENR SSQQPVSEVS TIPCPRIDTQ QLDRQIKAIM 300
KEVIPFLKEH MDEVCSSQLL TSVRRMVLTL TQQNDESKEF VKFFHKQLGS ILQDSLAKFA 360
GRKLKDCGED LLVEISEVLF NELAFFKLMQ DLDNNSITVK QRCKRKIEAT GVIQSCAKEA 420
KRILEDHGSP AGEIDDEDKD KDETETVKQT QTSEVYDGPK NVRSDISDQE EDEESEGCPV 480
SINLSKAETQ ALTNYGSGED ENEDEEMEEF EEGPVDVQTS LQANTEATEE NEHDEQVLQR 540
DFKKTAESKN VPLEREATSK NDQNNCPVKP CYLNILEDEQ PLNSAAHKES PPTVDSTQQP 600
NPLPLRLPEM EPLVPRVKEV KSAQETPESS LAGSPDTESP VLVNDYEAES GNISQKSDEE 660
DFVKVEDLPL KLTIYSEADL RKKMVEEEQK NHLSGEICEM QTEELAGNSE TLKEPETVGA 720
QSI 723 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA. 
Interpro
 IPR024138; Pericentriolar_Pcm1. 
Pfam
  
SMART
  
PROSITE
  
PRINTS