CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016983
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 T-lymphoma invasion and metastasis-inducing protein 2 
Protein Synonyms/Alias
 TIAM-2; SIF and TIAM1-like exchange factor 
Gene Name
 TIAM2 
Gene Synonyms/Alias
 KIAA2016; STEF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1254KPVQRVLKYPLLLKEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP- dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA. 
Sequence Annotation
 DOMAIN 506 620 PH 1.
 DOMAIN 810 881 RBD.
 DOMAIN 890 976 PDZ.
 DOMAIN 1099 1293 DH.
 DOMAIN 1347 1478 PH 2.
 MOD_RES 1648 1648 Phosphothreonine (By similarity).
 LIPID 2 2 N-myristoyl glycine (Potential).  
Keyword
 Alternative splicing; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Lipoprotein; Myristate; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1701 AA 
Protein Sequence
MGNSDSQYTL QGSKNHSNTI TGAKQIPCSL KIRGIHAKEE KSLHGWGHGS NGAGYKSRSL 60
ARSCLSHFKS NQPYASRLGG PTCKVSRGVA YSTHRTNAPG KDFQGISAAF STENGFHSVG 120
HELADNHITS RDCNGHLLNC YGRNESIAST PPGEDRKSPR VLIKTLGKLD GCLRVEFHNG 180
GNPSKVPAED CSEPVQLLRY SPTLASETSP VPEARRGSSA DSLPSHRPSP TDSRLRSSKG 240
SSLSSESSWY DSPWGNAGEL SEAEGSFLAP GMPDPSLHAS FPPGDAKKPF NQSSSLSSLR 300
ELYKDANLGS LSPSGIRLSD EYMGTHASLS NRVSFASDID VPSRVAHGDP IQYSSFTLPC 360
RKPKAFVEDT AKKDSLKARM RRISDWTGSL SRKKRKLQEP RSKEGSDYFD SRSDGLNTDV 420
QGSSQASAFL WSGGSTQILS QRSESTHAIG SDPLRQNIYE NFMRELEMSR TNTENIETST 480
ETAESSSESL SSLEQLDLLF EKEQGVVRKA GWLFFKPLVT VQKERKLELV ARRKWKQYWV 540
TLKGCTLLFY ETYGKNSMDQ SSAPRCALFA EDSIVQSVPE HPKKENVFCL SNSFGDVYLF 600
QATSQTDLEN WVTAVHSACA SLFAKKHGKE DTLRLLKNQT KNLLQKIDMD SKMKKMAELQ 660
LSVVSDPKNR KAIENQIQQW EQNLEKFHMD LFRMRCYLAS LQGGELPNPK SLLAAASRPS 720
KLALGRLGIL SVSSFHALVC SRDDSALRKR TLSLTQRGRN KKGIFSSLKG LDTLARKGKE 780
KRPSITQVDE LLHIYGSTVD GVPRDNAWEI QTYVHFQDNH GVTVGIKPEH RVEDILTLAC 840
KMRQLEPSHY GLQLRKLVDD NVEYCIPAPY EYMQQQVYDE IEVFPLNVYD VQLTKTGSVC 900
DFGFAVTAQV DERQHLSRIF ISDVLPDGLA YGEGLRKGNE IMTLNGEAVS DLDLKQMEAL 960
FSEKSVGLTL IARPPDTKAT LCTSWSDSDL FSRDQKSLLP PPNQSQLLEE FLDNFKKNTA 1020
NDFSNVPDIT TGLKRSQTDG TLDQVSHREK MEQTFRSAEQ ITALCRSFND SQANGMEGPR 1080
ENQDPPPRSL ARHLSDADRL RKVIQELVDT EKSYVKDLSC LFELYLEPLQ NETFLTQDEM 1140
ESLFGSLPEM LEFQKVFLET LEDGISASSD FNTLETPSQF RKLLFSLGGS FLYYADHFKL 1200
YSGFCANHIK VQKVLERAKT DKAFKAFLDA RNPTKQHSST LESYLIKPVQ RVLKYPLLLK 1260
ELVSLTDQES EEHYHLTEAL KAMEKVASHI NEMQKIYEDY GTVFDQLVAE QSGTEKEVTE 1320
LSMGELLMHS TVSWLNPFLS LGKARKDLEL TVFVFKRAVI LVYKENCKLK KKLPSNSRPA 1380
HNSTDLDPFK FRWLIPISAL QVRLGNPAGT ENNSIWELIH TKSEIEGRPE TIFQLCCSDS 1440
ESKTNIVKVI RSILRENFRR HIKCELPLEK TCKDRLVPLK NRVPVSAKLA SSRSLKVLKN 1500
SSSNEWTGET GKGTLLDSDE GSLSSGTQSS GCPTAEGRQD SKSTSPGKYP HPGLADFADN 1560
LIKESDILSD EDDDHRQTVK QGSPTKDIEI QFQRLRISED PDVHPEAEQQ PGPESGEGQK 1620
GGEQPKLVRG HFCPIKRKAN STKRDRGTLL KAQIRHQSLD SQSENATIDL NSVLEREFSV 1680
QSLTSVVSEE CFYETESHGK S 1701 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
 GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0005096; F:GTPase activator activity; IEA:Compara.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; TAS:ProtInc.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005057; F:receptor signaling protein activity; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR001478; PDZ.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR003116; Raf-like_ras-bd. 
Pfam
 PF00595; PDZ
 PF00169; PH
 PF00621; RhoGEF 
SMART
 SM00228; PDZ
 SM00233; PH
 SM00455; RBD
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50106; PDZ
 PS50003; PH_DOMAIN
 PS50898; RBD 
PRINTS