CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024258
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable acetyl-CoA acetyltransferase 
Protein Synonyms/Alias
 Acetoacetyl-CoA thiolase 
Gene Name
 MSMEG_4920, MSMEI_4793 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
187QKAAAAWKDGVFADEpupylation[1, 2]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657]
 [2] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 
Functional Description
  
Sequence Annotation
 ACT_SITE 84 84 Acyl-thioester intermediate (By
 ACT_SITE 345 345 Proton acceptor (By similarity).
 ACT_SITE 375 375 Proton acceptor (By similarity).
 CROSSLNK 187 187 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 Acyltransferase; Complete proteome; Isopeptide bond; Reference proteome; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 388 AA 
Protein Sequence
MIVAGARTPV GKLMGSLKDF SGTDLGAIAI RAALEKANVP ASMVEYVIMG QVLTAGAGQM 60
PARQAAVAAG IPWDVAALSI NKMCLSGIDA IALADQLIRA GEFDVIVAGG QESMSQAPHL 120
LPKSREGYKY GDATLVDHLA YDGLHDVFTD QPMGALTEQR NDVDKFTRAE QDEYAAQSHQ 180
KAAAAWKDGV FADEVVPVSI PQRKGDPIEF AEDEGIRANT TAESLAGLKP AFRKDGTITA 240
GSASQISDGA AAVIVMNKAK AEELGLTWLA EIGAHGVVAG PDSTLQSQPA NAIKKAITRE 300
GITVDQLDVI EINEAFAAVA LASTKELGVD PAKVNVNGGA IAIGHPIGMS GARIALHAAL 360
ELARRGSGYA VAALCGAGGQ GDALVLRR 388 
Gene Ontology
 GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IEA:EC. 
Interpro
 IPR002155; Thiolase.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr.
 IPR020615; Thiolase_acyl_enz_int_AS.
 IPR020610; Thiolase_AS.
 IPR020617; Thiolase_C.
 IPR020613; Thiolase_CS.
 IPR020616; Thiolase_N. 
Pfam
 PF02803; Thiolase_C
 PF00108; Thiolase_N 
SMART
  
PROSITE
 PS00098; THIOLASE_1
 PS00737; THIOLASE_2
 PS00099; THIOLASE_3 
PRINTS