CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004360
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 rRNA 2'-O-methyltransferase fibrillarin 
Protein Synonyms/Alias
 U3 small nucleolar RNA-associated protein NOP1; Nucleolar protein 1; U3 snoRNA-associated protein NOP1 
Gene Name
 NOP1 
Gene Synonyms/Alias
 LOT3; YDL014W; D2870 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
85GGARGGAKVVIEPHRacetylation[1]
85GGARGGAKVVIEPHRubiquitination[2]
102GVYIARGKEDLLVTKacetylation[1, 3]
109KEDLLVTKNMAPGESacetylation[3]
121GESVYGEKRISVEEPacetylation[3]
121GESVYGEKRISVEEPubiquitination[2]
130ISVEEPSKEDGVPPTubiquitination[2]
150VWNPFRSKLAAGIMGubiquitination[4]
299KLREERIKPLEQLTLubiquitination[2]
Reference
 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. 
Sequence Annotation
 REGION 8 83 RGG-box.
 REGION 22 42 RNA-binding RGG-box (By similarity).
 REGION 58 75 RNA-binding RGG-box (By similarity).
 REGION 179 180 S-adenosyl-L-methionine binding (By
 REGION 198 199 S-adenosyl-L-methionine binding (By
 REGION 223 224 S-adenosyl-L-methionine binding (By
 REGION 281 313 Helical (Potential).
 BINDING 150 150 S-adenosyl-L-methionine (By similarity).
 BINDING 243 243 S-adenosyl-L-methionine (By similarity).
 MOD_RES 8 8 Asymmetric dimethylarginine (Probable).
 MOD_RES 12 12 Asymmetric dimethylarginine (Probable).
 MOD_RES 16 16 Asymmetric dimethylarginine (Probable).
 MOD_RES 22 22 Asymmetric dimethylarginine (Probable).
 MOD_RES 26 26 Asymmetric dimethylarginine (Probable).
 MOD_RES 30 30 Asymmetric dimethylarginine (Probable).
 MOD_RES 34 34 Asymmetric dimethylarginine (Probable).
 MOD_RES 40 40 Asymmetric dimethylarginine (Probable).
 MOD_RES 44 44 Asymmetric dimethylarginine (Probable).
 MOD_RES 48 48 Asymmetric dimethylarginine (Probable).
 MOD_RES 52 52 Asymmetric dimethylarginine (Probable).
 MOD_RES 58 58 Asymmetric dimethylarginine (Probable).
 MOD_RES 62 62 Asymmetric dimethylarginine (Probable).
 MOD_RES 66 66 Asymmetric dimethylarginine (Probable).
 MOD_RES 70 70 Asymmetric dimethylarginine (Probable).
 MOD_RES 73 73 Asymmetric dimethylarginine (Probable).
 MOD_RES 81 81 Asymmetric dimethylarginine (Probable).  
Keyword
 Complete proteome; Direct protein sequencing; Methylation; Methyltransferase; Nucleus; Reference proteome; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 327 AA 
Protein Sequence
MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG 60
SRGGARGGSR GGRGGAAGGA RGGAKVVIEP HRHAGVYIAR GKEDLLVTKN MAPGESVYGE 120
KRISVEEPSK EDGVPPTKVE YRVWNPFRSK LAAGIMGGLD ELFIAPGKKV LYLGAASGTS 180
VSHVSDVVGP EGVVYAVEFS HRPGRELISM AKKRPNIIPI IEDARHPQKY RMLIGMVDCV 240
FADVAQPDQA RIIALNSHMF LKDQGGVVIS IKANCIDSTV DAETVFAREV QKLREERIKP 300
LEQLTLEPYE RDHCIVVGRY MRSGLKK 327 
Gene Ontology
 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0031428; C:box C/D snoRNP complex; IDA:SGD.
 GO:0032040; C:small-subunit processome; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008649; F:rRNA methyltransferase activity; IMP:SGD.
 GO:0000494; P:box C/D snoRNA 3'-end processing; IMP:SGD.
 GO:0008033; P:tRNA processing; IEA:InterPro. 
Interpro
 IPR000692; Fibrillarin.
 IPR020813; Fibrillarin_CS. 
Pfam
 PF01269; Fibrillarin 
SMART
  
PROSITE
 PS00566; FIBRILLARIN 
PRINTS
 PR00052; FIBRILLARIN.