CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021190
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-alpha-acetyltransferase 50 
Protein Synonyms/Alias
 N-acetyltransferase 13; N-acetyltransferase 5; hNAT5; N-acetyltransferase san homolog; hSAN; NatE catalytic subunit 
Gene Name
 NAA50 
Gene Synonyms/Alias
 MAK3; NAT13; NAT5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17DVTPHNIKQLKRLNQubiquitination[1, 2, 3, 4]
20PHNIKQLKRLNQVIFubiquitination[3, 5]
34FPVSYNDKFYKDVLEacetylation[6, 7]
34FPVSYNDKFYKDVLEubiquitination[1, 2, 3, 4, 5, 8, 9, 10]
37SYNDKFYKDVLEVGEacetylation[6, 7, 11]
37SYNDKFYKDVLEVGEubiquitination[1, 2, 3, 4, 10]
91RRLGIGTKMLNHVLNubiquitination[9, 12]
135GFEIIETKKNYYKRIubiquitination[2]
140ETKKNYYKRIEPADAacetylation[7]
152ADAHVLQKNLKVPSGacetylation[6]
152ADAHVLQKNLKVPSGubiquitination[1, 3, 4]
166GQNADVQKTDN****ubiquitination[2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Human Naa50p (Nat5/San) displays both protein N alpha- and N epsilon-acetyltransferase activity.
 Evjenth R, Hole K, Karlsen OA, Ziegler M, Arnesen T, Lillehaug JR.
 J Biol Chem. 2009 Nov 6;284(45):31122-9. [PMID: 19744929]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [11] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [12] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865
Functional Description
 Probable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity. 
Sequence Annotation
 DOMAIN 6 155 N-acetyltransferase.
 REGION 79 90 Coenzyme A binding.
 MOD_RES 12 12 Phosphothreonine.
 MOD_RES 34 34 N6-acetyllysine.
 MOD_RES 37 37 N6-acetyllysine.
 MOD_RES 110 110 Phosphotyrosine.  
Keyword
 3D-structure; Acetylation; Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 169 AA 
Protein Sequence
MKGSRIELGD VTPHNIKQLK RLNQVIFPVS YNDKFYKDVL EVGELAKLAY FNDIAVGAVC 60
CRVDHSQNQK RLYIMTLGCL APYRRLGIGT KMLNHVLNIC EKDGTFDNIY LHVQISNESA 120
IDFYRKFGFE IIETKKNYYK RIEPADAHVL QKNLKVPSGQ NADVQKTDN 169 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
 GO:0006474; P:N-terminal protein amino acid acetylation; TAS:HGNC. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR000182; GNAT_dom. 
Pfam
 PF00583; Acetyltransf_1 
SMART
  
PROSITE
 PS51186; GNAT 
PRINTS